"12" . . "Stanley, L. K." . . "Restrik\u010Dn\u011B-modifika\u010Dn\u00ED enzym Typu I EcoR124I je ATP-dependentn\u00ED endonukle\u00E1za, kter\u00E1 pou\u017E\u00EDv\u00E1 translokaci dsDNA ke \u0161t\u011Bpen\u00ED ve vzd\u00E1len\u00FDch nespecifick\u00FDch m\u00EDstech. Bioinformatick\u00E1 anal\u00FDza podjednotky HsdR EcoR124I a p\u0159\u00EDbuzn\u00FDch enzym\u016F Typu I uk\u00E1zala, \u017Ee krom\u011B principi\u00E1ln\u00EDch PD-(E/D)xK motiv\u016F I, II a III, je p\u0159\u00EDtomen tak\u00E9 motiv QxxxY charakteristick\u00FD pro nukle\u00E1zy skupiny RecB. Motiv QxxxY je um\u00EDst\u011Bn C-termin\u00E1ln\u011B za motivem III v oblasti p\u0159edpokl\u00E1dan\u00E9ho -helixu. Pou\u017Eit\u00EDm mutageneze byla zkoum\u00E1na \u00FAloha aa zbytk\u016F Q a Y ve vazb\u011B na DNA, v translokaci a \u0161t\u011Bpen\u00ED. Je diskutov\u00E1na role motivu QxxxY p\u0159i koordinaci katalytick\u00FDch zbytk\u016F nebo p\u0159i stabilizaci nukle\u00E1zov\u00E9 dom\u00E9ny na DNA"@cs . "A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I"@en . "0305-1048" . . "restriction endonuclease; mutagenesis; dsdna"@en . "2"^^ . "P(GA204/07/0325), Z(AV0Z50200510)" . "Nukle\u00E1zov\u00FD motiv RecB-rodiny u restrik\u010Dn\u00ED endonukle\u00E1zy Typu I EcoR124I"@cs . "4"^^ . "Weiserov\u00E1, Marie" . "354439" . "11"^^ . . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I" . . "A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I"@en . "\u0160i\u0161\u00E1kov\u00E1, Eva" . "RIV/61388971:_____/08:00311266" . . . "[79048207AF0C]" . "Nukle\u00E1zov\u00FD motiv RecB-rodiny u restrik\u010Dn\u00ED endonukle\u00E1zy Typu I EcoR124I"@cs . . . "A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I" . "Szczelkun, M. D." . "36" . . . "The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted -helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed"@en . . "Nucleic Acids Research" . . "RIV/61388971:_____/08:00311266!RIV09-AV0-61388971" . . . . "000257578600007" . "The Type I restriction-modification enzyme EcoR124I is an ATP-dependent endonuclease that uses dsDNA translocation to locate and cleave distant non-specific DNA sites. Bioinformatic analysis of the HsdR subunits of EcoR124I and related Type I enzymes showed that in addition to the principal PD-(E/D)xK Motifs, I, II and III, a QxxxY motif is also present that is characteristic of RecB-family nucleases. The QxxxY motif resides immediately C-terminal to Motif III within a region of predicted -helix. Using mutagenesis, we examined the role of the Q and Y residues in DNA binding, translocation and cleavage. Roles for the QxxxY motif in coordinating the catalytic residues or in stabilizing the nuclease domain on the DNA are discussed" . .