"colitis; animal model; inflammatory bowel disease"@en . "\u0160t\u011Bp\u00E1nkov\u00E1, Renata" . "The study was focused on the evaluation of intestinal microbiota on the development of intestinal inflammation in mice model. We used the model of chronic inflammation that develops spontaneously in the colon of conventional severe combined immunodeficiency (SCID) mice restored with the CD45 RBhigh subset of CD4+ T cells isolated from the spleen of immunocompetent BALB/c mice. Germ-free mice or mice monoassociated with Enterococcus faecalis, SFB (segmented filamentous bacteria), Fusobacterium mortiferum, Bacteroides distasonis and combination Fusobacterium mortiferum and SFB or Bacteroides distasonis a SFB were used as recipients. Mice were evaluated 8-12 weeks after cell transfer and severe colitis was present in conventional animals and in mice colonized with a cocktail of SPF microflora with SFB. Altered intestinal barrier in the terminal ileum of mice with severe colitis was documented by immunohistology using antibodies to ZO-1"@en . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "10" . "RIV/61388971:_____/07:00096944!RIV08-AV0-61388971" . "Koz\u00E1kov\u00E1, Hana" . . . . "The study was focused on the evaluation of intestinal microbiota on the development of intestinal inflammation in mice model. We used the model of chronic inflammation that develops spontaneously in the colon of conventional severe combined immunodeficiency (SCID) mice restored with the CD45 RBhigh subset of CD4+ T cells isolated from the spleen of immunocompetent BALB/c mice. Germ-free mice or mice monoassociated with Enterococcus faecalis, SFB (segmented filamentous bacteria), Fusobacterium mortiferum, Bacteroides distasonis and combination Fusobacterium mortiferum and SFB or Bacteroides distasonis a SFB were used as recipients. Mice were evaluated 8-12 weeks after cell transfer and severe colitis was present in conventional animals and in mice colonized with a cocktail of SPF microflora with SFB. Altered intestinal barrier in the terminal ileum of mice with severe colitis was documented by immunohistology using antibodies to ZO-1" . "Hudcovic, Tom\u00E1\u0161" . . "13" . "449195" . . . "Heczko, P." . . "1202;1211" . . . "Segmentovan\u00E9 filament\u00F3zn\u00ED bakterie p\u0159\u00EDtomn\u00E9 v definovan\u00E9m bakteri\u00E1ln\u00EDm koktejlu navozuj\u00ED st\u0159evn\u00ED z\u00E1n\u011Bt u my\u0161\u00ED po p\u0159enosu CD45 RBhigh CD4+T bun\u011Bk"@cs . "Segmented filamentous bacteria in defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RB(high) CD4+ T cell" . "Uhlig, H." . "Benada, Old\u0159ich" . "RIV/61388971:_____/07:00096944" . . . . . "[1467AE130FD3]" . . . "\u0158eh\u00E1kov\u00E1, Zuzana" . "Tlaskalov\u00E1, Helena" . "Segmentovan\u00E9 filament\u00F3zn\u00ED bakterie p\u0159\u00EDtomn\u00E9 v definovan\u00E9m bakteri\u00E1ln\u00EDm koktejlu navozuj\u00ED st\u0159evn\u00ED z\u00E1n\u011Bt u my\u0161\u00ED po p\u0159enosu CD45 RBhigh CD4+T bun\u011Bk"@cs . "Tato pr\u00E1ce byla zam\u011B\u0159ena na zhodnocen\u00ED vlivu st\u0159evn\u00EDch bakteri\u00ED na vznik z\u00E1n\u011Btu. V\u00FDvoj st\u0159evn\u00EDho z\u00E1n\u011Btu byl studov\u00E1n v chronick\u00E9m modelu, kdy po p\u0159enosu slezinn\u00FDch T bun\u011Bk z BALB/c my\u0161\u00ED do konven\u010Dn\u00EDch SCID my\u0161\u00ED, doch\u00E1z\u00ED ke vzniku spont\u00E1nn\u00EDho st\u0159evn\u00EDho z\u00E1n\u011Btu. Bezmikrobn\u00ED SCID my\u0161i byly monokolonizov\u00E1ny Enterococcus faecalis, Segmented fi lamentous bacteia (SFB), Fusobacterium mortiferum, Bacteroides distasonis a kombinac\u00ED Fusobacterium mortiferum a SFB nebo Bacteroides distasonis a SFB. SCID my\u0161i byly tak\u00E9 kolonizov\u00E1ny definovan\u00FDm koktejlem (SPF). Po 8-12 t\u00FDdnech byla kolitida nalezena u konven\u010Dn\u00EDch SCID my\u0161\u00ED a u my\u0161\u00ED, kter\u00E9 byly kolonizov\u00E1ny bakteri\u00E1ln\u00EDm koktejlem SPF a SFB. Zm\u011Bny st\u0159evn\u00ED bari\u00E9ry v termin\u00E1ln\u00EDm ileu byly detekov\u00E1ny imunohistochemicky pomoc\u00ED protil\u00E1tky ZO-1"@cs . "1078-0998" . . . "Segmented filamentous bacteria in defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RB(high) CD4+ T cell"@en . "Segmented filamentous bacteria in defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RB(high) CD4+ T cell"@en . . "Inflammatory Bowel Diseases" . "11"^^ . "Segmented filamentous bacteria in defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RB(high) CD4+ T cell" . . "P(1QS500200572), P(GA303/06/0974), P(IAA5020205), Z(AV0Z50200510)" . "Kofro\u0148ov\u00E1, Olga" . . "14"^^ . . . . "8"^^ . "Strus, M." . "Powrie, F." . . "Hrn\u010D\u00ED\u0159, Tom\u00E1\u0161" . "Read, S." . "Bland, P." .