"Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis"@en . "P(IAA600660607), Z(AV0Z50200510)" . . "Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract\u2013(NH4)2SO4\u2013glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium" . . "RIV/61388971:_____/07:00096764" . "Glukosylglycer\u00E1t je osmolit a extracelul\u00E1rn\u00ED metabolit produkovan\u00FD Streptomyces caelestis"@cs . . "Folia Microbiologica" . "Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis"@en . . . "Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis" . "[CE726D1D94D9]" . . . "Pet\u0159\u00ED\u010Dek, Miroslav" . "6"^^ . . . . "streptomyces caelestis; mass spectrometry"@en . . . . "Streptomyces caelestis DSM 40 084 produces two osmolytes, viz. 2-O-(D-glucopyranosyl)-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3 procent NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells, its concentration reached 3 g/L during the cultivation in a yeast extract\u2013(NH4)2SO4\u2013glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium"@en . "423486" . "Halada, Petr" . . . "451;456" . "Posp\u00ED\u0161il, Stanislav" . "Glukosylglycer\u00E1t je osmolit a extracelul\u00E1rn\u00ED metabolit produkovan\u00FD Streptomyces caelestis"@cs . . "52" . "CZ - \u010Cesk\u00E1 republika" . "5" . . "Streptomyces caelestis DSM 40 084 produkuje dva osmolyty: 2-O-(D-glukopyranosyl-glycerolovou kyselinu (GG) a trehalosu. Ob\u011B slou\u010Deniny byly izolov\u00E1ny a identifikov\u00E1ny nukle\u00E1rn\u00ED magnetickou resonanc\u00ED a hmotnostn\u00ED spektrometri\u00ED. Byla zji\u0161t\u011Bna velmi citliv\u00E1 regulace bun\u011B\u010Dn\u00FDch osmolyt\u016F v exponenci\u00E1ln\u011B rostouc\u00EDch kultur\u00E1ch. Intracelul\u00E1rn\u00ED obsah GG a trehalosy se zv\u00FD\u0161il dvakr\u00E1t po skokov\u00E9 zm\u011Bn\u011B osmolarity m\u00E9dia vyvolan\u00E9 0.3 procent NaCl. 1H NMR anal\u00FDza bun\u011B\u010Dn\u00FDch extrakt\u016F nepotvrdila p\u0159\u00EDtomnost dal\u0161\u00EDch osmolyt\u016F. GG je metabolit uvol\u0148ovan\u00FD z bun\u011Bk a jeho koncentrace dosahuje 3g/L p\u0159i kultivaci v m\u00E9diu obsahuj\u00EDc\u00ED yeast extrakt, (NH4)2SO4 a glycerol. Tato pr\u00E1ce poprv\u00E9 informuje o v\u00FDskytu iontov\u00E9ho osmolytu GG u rodu Streptomyces a o jeho voln\u00E9 exkreci do m\u00E9dia"@cs . . "RIV/61388971:_____/07:00096764!RIV08-AV0-61388971" . . "Sedmera, Petr" . "4"^^ . "0015-5632" . "4"^^ . "Glucosylglycerate Is an Osmotic Solute and an Extracellular Metabolite Produced by Streptomyces caelestis" .