"Kysl\u00EDk, Pavel" . "413924" . "Klonov\u00E1n\u00ED genu k\u00F3duj\u00EDc\u00EDho epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinov\u00FDm in\u017Een\u00FDrstv\u00EDm"@cs . . . "Kot\u00EDk, Michael" . "Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering"@en . . "The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site"@en . "[992DFE955C7C]" . . . . "Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering"@en . . . . "8"^^ . "8;15" . . "-" . "Mare\u0161ov\u00E1, Helena" . "RIV/61388971:_____/07:00090142" . . . "Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering" . . "Klonov\u00E1n\u00ED genu k\u00F3duj\u00EDc\u00EDho epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinov\u00FDm in\u017Een\u00FDrstv\u00EDm"@cs . "P(GA204/06/0458), Z(AV0Z50200510)" . "Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering" . "132" . "4"^^ . . "RIV/61388971:_____/07:00090142!RIV08-AV0-61388971" . . "4"^^ . "0168-1656" . "protein engineering; saturation mutagenesis; enantioselectivity"@en . "\u0160t\u011Bp\u00E1nek, V\u00E1clav" . "NL - Nizozemsko" . . . . . . "Gen k\u00F3duj\u00EDc\u00ED epoxidhydrolasu z Aspergillus niger M200 byl klonov\u00E1n. Satura\u010Dn\u00ED mutageneze kodonu 217 vedla k objeven\u00ED dev\u00EDti variant enzymu (v n\u011Bkter\u00FDch p\u0159\u00EDpadech) s v\u00FDrazn\u00FDmi rozd\u00EDly v aktivit\u011B a enantioselektivit\u011B (po porovn\u00E1n\u00ED s p\u016Fvodn\u00EDm nezm\u011Bn\u011Bn\u00FDm enzymem). Pozice 217 je lokalizov\u00E1na u vchodu do substr\u00E1tov\u00E9ho tunelu, kter\u00FD spojuje aktivn\u00ED centrum enzymu s vn\u011Bj\u0161kem"@cs . "The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site" . "Journal of Biotechnology" .