"Reisnerov\u00E1, H." . "The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma, the R5-28 cells were cultivated in vitro and then both the supernatants nad the cell lysates were analysed. Our experiments showed three cytokines to be produced by R5-28 cell in vitro. Moreover, in vivo, other three cytokines were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed." . "Multiplex analysis of cytokines involved in tumour growth and spontaneous regression in a rat sarcoma model" . . "Usvald, Du\u0161an" . . "J\u00EDlek, F." . . "Folia Biologica" . . . "1"^^ . "Pl\u00E1nsk\u00E1, Daniela" . "cytokines; sarcoma cells; spontaneous regression"@en . . "Multiplex analysis of cytokines involved in tumour growth and spontaneous regression in a rat sarcoma model" . . . "RIV/61388971:_____/07:00089246" . "RIV/61388971:_____/07:00089246!RIV08-AV0-61388971" . "0015-5500" . . "53" . . . "435704" . . "Vannucci, Luca" . "Strn\u00E1del, J\u00E1n" . "The aim of our study was to examine in vivo and in vitro cytokines produced by Lewis rat derived R5-28 sarcoma cells. These cells produce rapidly growing tumours in approximately two weeks after subcutaneous inoculation. However, spontaneous tumour regression was noted in about 40% of animals. For an explanation of this phenomenon, we evaluated the profile of 19 cytokines during tumour growth and spontaneous regression by the use of antibody array. To detect cytokines directly originated by the sarcoma, the R5-28 cells were cultivated in vitro and then both the supernatants nad the cell lysates were analysed. Our experiments showed three cytokines to be produced by R5-28 cell in vitro. Moreover, in vivo, other three cytokines were detected both in blood sera and tumour lysates, probably produced by immune and stromal cells during tumour growth. Changes in their expression after spontaneous regression are discussed."@en . . "P(2B06130), P(GA524/04/0102), P(GD310/03/H147), P(GD523/03/H076), P(IAA500200510), P(IAA600450601), Z(AV0Z50200510), Z(AV0Z50450515), Z(MSM6046070901)" . "Hor\u00E1k, Vratislav" . "V\u00E1\u0148a, Petr" . "4"^^ . "216;219" . . . "Kverka, Miloslav" . . . "V p\u0159edlo\u017Een\u00E9 pr\u00E1ci bylo na\u0161\u00EDm c\u00EDlem charakterizovat zm\u011Bny v expresi cytok\u00EDn\u016F b\u011Bhem r\u016Fstu a p\u0159i spont\u00E1nn\u00ED regresi n\u00E1dor\u016F na vlastn\u00EDm n\u00E1dorov\u00E9m modelu. Bu\u0148ky linie R5-28 jsou schopn\u00E9 indukovat rychle rostouc\u00ED n\u00E1dory v pr\u016Fb\u011Bhu dvou t\u00FDdn\u016F po podko\u017En\u00ED inokulaci u potkan\u016F l\u00EDnie Lewis. P\u0159ibli\u017En\u011B u 40% testovan\u00FDch zv\u00ED\u0159at je mo\u017En\u00E9 pozorovat spont\u00E1nn\u00ED regresi n\u00E1dor\u016F. \u00DAlohu imunitn\u00EDho syst\u00E9mu a cytok\u00EDn\u016F jako modul\u00E1tor\u016F imunitn\u00ED odpov\u011Bdi na n\u00E1dor jsme v tomto procesu testovali pou\u017Eit\u00EDm \u201Eantibody array\u201C \u2013 techniky, umo\u017E\u0148uj\u00EDc\u00ED simult\u00E1nn\u00ED detekci exprese 19 cytok\u00EDn\u016F. K rozli\u0161en\u00ED cytok\u00EDn\u016F produkovan\u00FDch p\u0159\u00EDmo sarkomov\u00FDmi bu\u0148kami R5 \u2013 28 od cytok\u00EDn\u016F, produkovan\u00FDch bu\u0148kami n\u00E1dorov\u00E9ho mikroprost\u0159ed\u00ED jsme testovali expresi cytok\u00EDn\u016F v kultiva\u010Dn\u00EDm m\u00E9diu pro R5 \u2013 28, lyz\u00E1t\u011B bun\u011Bk linie R5 \u2013 28, v celkov\u00E9m n\u00E1dorov\u00E9m lyz\u00E1t\u011B a v s\u00E9ru pokusn\u00FDch zv\u00ED\u0159at. Na\u0161e experimenty uk\u00E1zali, \u017Ee bu\u0148ky linie R5 \u2013 28 produkuj\u00ED in vitro t\u0159i druhy cytok\u00EDn\u016F \u2013 MCP \u2013 1, TIMP \u2013 1 a VEGF. Typ produkovan\u00FDch cytok\u00EDn\u016F koreluje s n\u011Bkter\u00FDmi charakteristi..."@cs . "Hlu\u010Dilov\u00E1, Jana" . "10"^^ . "Multiplexn\u00ED anal\u00FDza cytok\u00EDn\u016F p\u0159i progresi a spont\u00E1nn\u00ED regresi n\u00E1doru na sarkomov\u00E9m modelu potkana"@cs . "Multiplex analysis of cytokines involved in tumour growth and spontaneous regression in a rat sarcoma model"@en . . "Multiplex analysis of cytokines involved in tumour growth and spontaneous regression in a rat sarcoma model"@en . . "[BFE408B3758B]" . . . . "Multiplexn\u00ED anal\u00FDza cytok\u00EDn\u016F p\u0159i progresi a spont\u00E1nn\u00ED regresi n\u00E1doru na sarkomov\u00E9m modelu potkana"@cs . "CZ - \u010Cesk\u00E1 republika" . . "-" . .