"HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets" . "\u0160ebo, Peter" . "10"^^ . "Hejnov\u00E1, Jana" . "Felsberg, J\u00FCrgen" . "12"^^ . . . . . "escherichia coli; haemolysin; colonization"@en . "Faldyna, M." . "Kmen Escherichia coli A0 34/86 (O83:K24:H31) je \u00FAsp\u011B\u0161n\u011B pou\u017E\u00EDv\u00E1n pro preventivn\u00ED kolonizaci novorozenc\u016F. V t\u00E9to pr\u00E1ci ukazujeme, \u017Ee jeho bezpe\u010Dnost lze je\u0161t\u011B vylep\u0161it odstran\u011Bn\u00EDm genu pro hemolyz\u00EDn, co\u017E neovlivn\u00ED koloniza\u010Dn\u00ED schopnosti kmene v prase\u010D\u00EDm experiment\u00E1ln\u00EDm modelu"@cs . . "Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the a-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks"@en . "[74EA50282A6F]" . . "Escherichia coli A0 34/86 (O83:K24:H31) has been successfully used for prophylactic and therapeutic intestinal colonization of premature and newborn infants, with the aim of preventing nosocomial infections. Although E. coli A0 34/86 was described as a nonpathogenic commensal, partial sequencing revealed that its genome harbours gene clusters highly homologous to virulence determinants of different types of E. coli, including closely linked genes of the a-haemolysin operon (hlyCABD) and for the cytotoxic necrotizing factor (cnf1). A haemolysin-deficient mutant (hlyA) of E. coli A0 34/86 was generated and its colonization capacity was determined. The results show that a single dose of the A0 34/86 wild-type or hlyA strains resulted in efficient intestinal colonization of newborn conventional piglets, and that this was still considerable after several weeks" . "NL - Nizozemsko" . "0928-8244" . "Sheshko, Valeria" . "257;266" . . "Vy\u0159azen\u00ED HlyA vede k bezpe\u010Dn\u011Bj\u0161\u00ED variant\u011B kmene Escherichia coli A0 34/86 s nezm\u011Bn\u011Bn\u00FDmi koloniza\u010Dn\u00EDmi schopnostmi"@cs . . "Sheshko, Valeria" . . "Vy\u0159azen\u00ED HlyA vede k bezpe\u010Dn\u011Bj\u0161\u00ED variant\u011B kmene Escherichia coli A0 34/86 s nezm\u011Bn\u011Bn\u00FDmi koloniza\u010Dn\u00EDmi schopnostmi"@cs . . "\u0158eh\u00E1kov\u00E1, Z." . "RIV/61388971:_____/06:00043244" . "Alexa, P." . "HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets"@en . . . "Bomba, A." . . "FEMS Immunology and Medical Microbiology" . "P(GA523/03/0186), Z(AV0Z50200510)" . . "\u0160inkora, J." . "4"^^ . "-" . "N\u011Bmcov\u00E1, R." . . . "477891" . . "HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets" . "HlyA knock out yields a safer Escherichia coli A0 34/86 variant with unaffected colonization capacity in piglets"@en . "48" . . . "RIV/61388971:_____/06:00043244!RIV07-GA0-61388971" .