"Pou\u017Eili jsme neacylovan\u00FD proCyaA, kter\u00FD netvo\u0159\u00ED membr\u00E1nov\u00E9 p\u00F3ry, a specifick\u00E9 CyaA mutanty vykazuj\u00EDc\u00ED selektivn\u011B sn\u00ED\u017Eenou hemolytickou aktivitu, ale maj\u00EDc\u00ED plnou schopnost zvy\u0161ovat cAMP v bu\u0148k\u00E1ch, k podpo\u0159en\u00ED pozorov\u00E1n\u00ED, \u017Ee enzymatick\u00E1 deplece bun\u011B\u010Dn\u00E9ho ATP d\u00EDky akci toxinu je dosta\u010Duj\u00EDc\u00ED ke zp\u016Fsoben\u00ED bun\u011B\u010Dn\u00E9 smrti a bun\u011B\u010Dn\u00E9 lyze. D\u00E1le jsme uk\u00E1zali, \u017Ee hemolytick\u00E1 aktivita CyaA synergizuje s invazivn\u00ED AC aktivitou a t\u00EDm maximalizuje celkovou cytolytickou aktivitu toxinu"@cs . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "Ma\u0161\u00EDn, Ji\u0159\u00ED" . . . . "Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes"@en . "74" . . "P(1M0506), P(IAA5020406), Z(AV0Z50200510)" . "RIV/61388971:_____/06:00040415!RIV07-MSM-61388971" . "Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes" . "8"^^ . . . "We used here the non-acylated proCyaA, unable to form membrane pores, and the specific CyaA mutants exhibiting a selectively reduced hemolytic activity but full capacity to elevate cAMP in cells, to corroborate the observation that enzymatic depletion of cellular ATP due to toxin action is sufficient to promote cell death and lysis. It is further shown, that pore-forming (hemolytic) activity of CyaA synergizes with the invasive AC enzyme activity in maximizing the overall cytolytic potency of the toxin" . "Kan\u00E1lotvorn\u00E1 a enzymatick\u00E1 aktivita Adenyl\u00E1t cykl\u00E1zov\u00E9ho toxinu Bordetella pertussis synergizuj\u00ED v p\u016Fsoben\u00ED lyze monocyt\u016F"@cs . "Kan\u00E1lotvorn\u00E1 a enzymatick\u00E1 aktivita Adenyl\u00E1t cykl\u00E1zov\u00E9ho toxinu Bordetella pertussis synergizuj\u00ED v p\u016Fsoben\u00ED lyze monocyt\u016F"@cs . . "\u0160ebo, Peter" . . . . "bordetella pertussis; cyaa; cytotoxicity"@en . "We used here the non-acylated proCyaA, unable to form membrane pores, and the specific CyaA mutants exhibiting a selectively reduced hemolytic activity but full capacity to elevate cAMP in cells, to corroborate the observation that enzymatic depletion of cellular ATP due to toxin action is sufficient to promote cell death and lysis. It is further shown, that pore-forming (hemolytic) activity of CyaA synergizes with the invasive AC enzyme activity in maximizing the overall cytolytic potency of the toxin"@en . "Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes"@en . . "2207;2214" . . "[D9EC778CE1C6]" . "Infection and Immunity" . "5" . "Basler, Marek" . "492988" . . . . . "0019-9567" . "4"^^ . . "Pore-Forming and Enzymatic Activities of Bordetella pertussis Adenylate Cyclase Toxin Synergize in Promoting Lysis of Monocytes" . "Osi\u010Dka, Radim" . . . "4"^^ . "RIV/61388971:_____/06:00040415" .