. . "Adenyl\u00E1tcykl\u00E1zov\u00FD toxin bakterie Bordetella indukuje kask\u00E1du morfologick\u00FDch zm\u011Bn ov\u010D\u00EDch erytrocyt\u016F a nach\u00E1z\u00ED se ve shluc\u00EDch v erytrocyt\u00E1rn\u00ED membr\u00E1n\u011B"@cs . . . . . "11"^^ . "RIV/61388971:_____/06:00040411!RIV07-AV0-61388971" . "Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes"@en . "Adenyl\u00E1tcykl\u00E1zov\u00FD toxin bakterie Bordetella indukuje kask\u00E1du morfologick\u00FDch zm\u011Bn ov\u010D\u00EDch erytrocyt\u016F a nach\u00E1z\u00ED se ve shluc\u00EDch v erytrocyt\u00E1rn\u00ED membr\u00E1n\u011B"@cs . "Kofro\u0148ov\u00E1, Olga" . "4"^^ . . "RIV/61388971:_____/06:00040411" . "Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes"@en . "This research article describes effects of Adenylate cyclase toxin (CyaA) of Bordetella pertussis on morphology of sheep erythrocytes and its localization in erythrocyte membranes. CyaA was shown to induce a cascade of morphological changes of erythrocytes, such as shrinkage, formation of membrane projections, and blebs and swelling. Further, immunolabeling of inserted CyaA-232/FLAG molecules with specific anti-FLAG antibodies and IgG-gold particles indicated a clustered distribution of CyaA molecules in erythrocyte membrane. This was confirmed by immunofluorescence and confocal microscopy, which revealed uniform stoichiometry of CyaA clusters, suggesting CyaA binding into specific domains in erythrocyte membrane" . "119;129" . . "[FA88E917EA1E]" . "P(IAA5020406), Z(AV0Z50200510)" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Vojtov\u00E1, Jana" . "4"^^ . . . . "This research article describes effects of Adenylate cyclase toxin (CyaA) of Bordetella pertussis on morphology of sheep erythrocytes and its localization in erythrocyte membranes. CyaA was shown to induce a cascade of morphological changes of erythrocytes, such as shrinkage, formation of membrane projections, and blebs and swelling. Further, immunolabeling of inserted CyaA-232/FLAG molecules with specific anti-FLAG antibodies and IgG-gold particles indicated a clustered distribution of CyaA molecules in erythrocyte membrane. This was confirmed by immunofluorescence and confocal microscopy, which revealed uniform stoichiometry of CyaA clusters, suggesting CyaA binding into specific domains in erythrocyte membrane"@en . . . . . "467295" . "V tomto \u010Dl\u00E1nku je pops\u00E1n efekt adenyl\u00E1tcykl\u00E1zov\u00E9ho toxinu (CyaA) bakterie Bordetella pertusis na morfologii ov\u010D\u00EDch erytrocyt\u016F a jeho lokalizace v membr\u00E1n\u011B erytrocyt\u016F. Bylo uk\u00E1z\u00E1no, \u017Ee CyaA indukuje postupnou zm\u011Bnu morfologie erytrocyt\u016F, je\u017E zahrnuje smr\u0161t\u011Bn\u00ED bun\u011Bk, tvorbu v\u00FDb\u011B\u017Ek\u016F a v\u00E1\u010Dk\u016F a bobtn\u00E1n\u00ED bun\u011Bk. D\u00E1le, imunodetekce zano\u0159en\u00FDch molekul CyaA-232/FLAG pomoc\u00ED specifick\u00E9 protil\u00E1tky anti-FLAG a sekund\u00E1rn\u00ED protil\u00E1tky s nav\u00E1zanou partikul\u00ED koloidn\u00EDho zlata uk\u00E1zala, \u017Ee molekuly CyaA se nach\u00E1zej\u00ED v membr\u00E1n\u011B ve shluc\u00EDch. Pomoc\u00ED konfok\u00E1ln\u00ED mikroskopie byla d\u00E1le zji\u0161t\u011Bna uniformn\u00ED stechiometrie shluk\u016F molekul CyaA, co\u017E nazna\u010Dilo mo\u017Enost, \u017Ee se molekuly CyaA v\u00E1\u017E\u00ED do specifick\u00FDch oblast\u00ED v membr\u00E1n\u011B erytrocyt\u016F"@cs . . "Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes" . "cyaa; scanning electron microscopy; transmission electron microscopy"@en . . "Microscopy Research Technique" . . . . "\u0160ebo, Peter" . "1059-910X" . "-" . "Benada, Old\u0159ich" . "Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes" . "69" .