. "We constructed a reporter gene system, based on luciferase genes from Vibrio harvei, for measurement of translation nonsense suppression in Streptomyces. Streptomyces lividans TK 24 was transformed with the plasmids and the reporter system was tested by growth of the strain in the presence of streptomycin as a translation accuracy modulator. Streptomycin increased nonsense suppression on UAA nearly 10-fold and more than 20-fold on UAG. Whereas on UGA, the most frequent stop signal in Streptomyces, the effect was negligible"@en . . "Sestrojili jsme genov\u00FD reporterov\u00FD syst\u00E9m vyu\u017E\u00EDvaj\u00EDc\u00ED lucifer\u00E1zu z Vibrio harvei pro m\u011B\u0159en\u00ED transla\u010Dn\u00ED nonsense suprese u streptomycet. Transformovali jsme Streptomyces lividans TK 24 plasmidem nesouc\u00EDm reporterov\u00FD syst\u00E9m, kter\u00FD jsme testovali b\u011Bhem r\u016Fstu kultury v p\u0159\u00EDtomnosti a nep\u0159\u00EDtomnosti streptomycinu jako modul\u00E1toru p\u0159esnosti translace. Streptomycin zvy\u0161oval nonsense supresi UAA t\u00E9m\u011B\u0159 desetkr\u00E1t a v p\u0159\u00EDpad\u011B UAG v\u00EDce ne\u017E dvacetkr\u00E1t. V p\u0159\u00EDpad\u011B UGA, nej\u010Dast\u011Bj\u0161\u00EDho stop sign\u00E1lu u streptomycet, byl efekt zanedbateln\u00FD"@cs . "Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces"@en . "Konstrukce a testov\u00E1n\u00ED reporterov\u00E9ho syst\u00E9mu, zalo\u017Een\u00E9ho na bakteri\u00E1ln\u00ED lucifer\u00E1ze, ur\u010Den\u00E9ho pro m\u011B\u0159en\u00ED nonsense suprese u streptomycet"@cs . . "469654" . "RIV/61388971:_____/06:00039435" . . . . . "3"^^ . "1" . . . "streptomyces; reporter gene system"@en . "[4645302A0BC2]" . "CZ - \u010Cesk\u00E1 republika" . "RIV/61388971:_____/06:00039435!RIV07-GA0-61388971" . . . "P(GA310/03/0292), Z(AV0Z50200510)" . "4"^^ . . . "62;64" . . "Branny, Pavel" . . "Folia Microbiologica" . "Konstrukce a testov\u00E1n\u00ED reporterov\u00E9ho syst\u00E9mu, zalo\u017Een\u00E9ho na bakteri\u00E1ln\u00ED lucifer\u00E1ze, ur\u010Den\u00E9ho pro m\u011B\u0159en\u00ED nonsense suprese u streptomycet"@cs . "Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces" . . "Weiser, Jaroslav" . . "5"^^ . . "51" . . "Kalachov\u00E1, Ladislava" . "We constructed a reporter gene system, based on luciferase genes from Vibrio harvei, for measurement of translation nonsense suppression in Streptomyces. Streptomyces lividans TK 24 was transformed with the plasmids and the reporter system was tested by growth of the strain in the presence of streptomycin as a translation accuracy modulator. Streptomycin increased nonsense suppression on UAA nearly 10-fold and more than 20-fold on UAG. Whereas on UGA, the most frequent stop signal in Streptomyces, the effect was negligible" . "Buri\u00E1nkov\u00E1, Karol\u00EDna" . "Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces"@en . "0015-5632" . "Construction and Testing of a Bacterial Luciferase Reporter Gene System for in Vivo Measurement of Nonsense Suppression in Streptomyces" . "Pernodet, J.-L." .