"2"^^ . "Mart\u00EDnkov\u00E1, Ludmila" . . "Lemaire, M." . "9"^^ . "467148" . "Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats" . "Cel\u00E9 bu\u0148ky bakt\u00E9rie Rhodococcus equi A4, producenta nitrilhydratasy a amidasy, byly imobilizov\u00E1ny v \u010Do\u010Dkovit\u00FDch \u010D\u00E1stic\u00EDch hydrogelu, LentiKats (R). Imobilizovan\u00FD biokatalyz\u00E1tor byl pou\u017Eit pro biotransformaci benzonitrilu, 3-kyanopyridinu, (R,S)-3-hydroxy-2-methylenebutanenitrilu and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrilu. Stabilita nitrilhydratasy b\u011Bhem opakovan\u00E9ho pou\u017Eit\u00ED biokatalyz\u00E1toru z\u00E1visela na typu substr\u00E1tu. Enzym byl stabiln\u00ED b\u011Bhem transformace (R,S)-3-hydroxy-2-methylenebutanenitrilu. B\u011Bhem reakce nebyla pozorov\u00E1na v\u00FDrazn\u011Bj\u0161\u00ED ztr\u00E1ta aktivity amidasy"@cs . . . "\u010Cejkov\u00E1, A." . "Biotransformace nitril\u016F pomoc\u00ED bakt\u00E9rie Rhodococcus equi A4 imobilizovan\u00E9 v LentiKats"@cs . "Mas\u00E1k, J." . "[3E220A3AC19D]" . "Journal of Molecular Catalysis B-Enzymatic" . "1381-1177" . "Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats"@en . . . . "Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats" . "Bolte, J." . "Biotransformation of nitriles by Rhodococcus equi A4 immobilized in LentiKats"@en . "NL - Nizozemsko" . "Kub\u00E1\u010D, David" . . "39" . . "Jirk\u016F, V." . "Biotransformace nitril\u016F pomoc\u00ED bakt\u00E9rie Rhodococcus equi A4 imobilizovan\u00E9 v LentiKats"@cs . "Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles. LentiKats (R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylenebutanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was most stable during the transformation of (R,S)-3-hydroxy-2-methylenebutanenitrile. No significant loss of the amidase activity was observed within the course of the biocatalytic reaction" . "nitrile hydratase; amidase; nitriles"@en . "RIV/61388971:_____/06:00039333!RIV07-GA0-61388971" . . . "59;61" . "Stloukal, R." . . "RIV/61388971:_____/06:00039333" . "-" . . . "Whole cells of Rhodococcus equi A4, a producer of nitrile hydratase and amidase activities, were immobilized in lens-shaped hydrogel particles. LentiKats (R). The immobilized biocatalyst was applied to the biotransformation of benzonitrile, 3-cyanopyridine, (R,S)-3-hydroxy-2-methylenebutanenitrile and (R,S)-3-hydroxy-2-methylene-3-phenylpropanenitrile. The stability of the nitrile hydratase during the repeated use of the biocatalyst was dependent on the type of the substrate. The enzyme was most stable during the transformation of (R,S)-3-hydroxy-2-methylenebutanenitrile. No significant loss of the amidase activity was observed within the course of the biocatalytic reaction"@en . "Gallienne, E." . . "P(GA203/05/2267), P(IAA4020213), P(OC D25.001), Z(AV0Z50200510)" . . "3"^^ . . . . . .