"Generalized polymer effective charge measurement by capillary isotachophoresis"@en . "Ka\u0161i\u010Dka, V\u00E1clav" . "Besson, A." . "000345724800029" . "I, P(7AMB12FR012), P(GA13-17224S)" . "1370" . . "5"^^ . . "In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range." . "NL - Nizozemsko" . "Chamieh, J." . "Generalized polymer effective charge measurement by capillary isotachophoresis" . "0021-9673" . "RIV/61388963:_____/14:00435675!RIV15-GA0-61388963" . "RIV/61388963:_____/14:00435675" . . "Nov 28" . "In this work, we have generalized the use of capillary isotachophoresis as a universal method for determination of effective charge of anionic and cationic (co)polymers on ordinary capillary electrophoresis instruments. This method is applicable to a broad range of strong or weak polyelectrolytes with good repeatability. Experimental parameters (components and concentrations of leading and terminating electrolytes, capillary diameters, constant electric current intensity) were optimized for implementation in 100 mu m i.d. capillaries for both polyanions and polycations. Determined values of polymer effective charge were in a very good agreement with those obtained by capillary electrophoresis with indirect UV detection. Uncertainty of the effective charge measurement using isotachophoresis was addressed and estimated to be similar to 5-10% for solutes with mobilities in the 20-50 x 10(-9) m(2) V-1 s(-1) range."@en . . . "Generalized polymer effective charge measurement by capillary isotachophoresis"@en . "2"^^ . "18042" . . "Generalized polymer effective charge measurement by capillary isotachophoresis" . "polymer effective charge; polyelectrolyte; isotachophoresis; counter-ion condensation; capillary electrophoresis"@en . . . . "10.1016/j.chroma.2014.10.025" . . . . "Cottet, H." . . . . . "Journal of Chromatography A" . "Koval, Du\u0161an" . "[A81952A3E3BD]" . . "8"^^ . . . .