"[27690922EFA1]" . . "2" . "Lyngstadaas, S. P." . "10.1166/jbt.2011.1018" . "Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation."@en . . "Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells"@en . "Vondr\u00E1\u0161ek, Ji\u0159\u00ED" . "1" . "000312570600009" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells" . "Regulation of biomineralization processes are mediated by extracellular matrix proteins that often exhibit proline-rich regions. Advanced bioinformatic methods were used to design the structure of artificial peptides (P1, P2, P3) based on proline-rich domains of extracellular proteins. Their effect on osteoblast differentiation and in vitro biomineralization was tested on MC3T3-E1 and human umbilical cord mesenchymal stem cells (hUCMSCs) and compared to the commercially available enamel matrix derivative (EMD). MC3T3-E1 and hUCMSCs treated with the synthetic peptides showed a decreased cytotoxicity after 24-48 h of treatment compared to control. MC373-E1 cells treated with EMD showed lower expression of osteoblast markers genes than cells treated with P2, except for collagen type I. In hUCMSCs, OC gene expression was higher in P2-treated cells compared to those treated with EMD or control. ALP activity was markedly increased in MC3T3-E1 cells incubated with P2 compared to other treatments. Similar results were observed in hUCMSCs. Further, P2 increased calcium deposition rate compared to EMD or control either in MC3T3-E1 or hUCMSCs. The observed effects of proline-rich peptides hold potential for both clinical applications and as a research tool in further investigations of the molecular basis of induced osteogenic cell differentiation." . . "233939" . . "Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells" . . . "RIV/61388963:_____/11:00389610!RIV13-AV0-61388963" . "proline-rich regions; synthetic peptides; bone formation; mineralization; In Vitro"@en . . . "Monjo, M." . . "Journal of Biomaterials and Tissue Engineering" . "Z(AV0Z40550506)" . . "2157-9083" . . . "Gaya, A." . . "1"^^ . "Synthetic Peptides Analogue to Enamel Proteins Promote Osteogenic Differentiation of MC3T3-E1 and Mesenchymal Stem Cells"@en . . . "12"^^ . . "RIV/61388963:_____/11:00389610" . "Ramis, J. M." . . "Rubert, M." . . "6"^^ .