"000297462400006" . "0961-8368" . . "5"^^ . "RIV/61388963:_____/11:00369816!RIV12-AV0-61388963" . . . . . . "RIV/61388963:_____/11:00369816" . . "Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall" . . "9"^^ . . "We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates." . "Vinterov\u00E1, Zuzana" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates."@en . "Candida parapsilosis; secreted aspartic proteinases; Sapp1p; cell wall; biotin; proteolytic activity"@en . "12" . "20" . . "Dost\u00E1l, Ji\u0159\u00ED" . . . . . "Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall" . . "Pichov\u00E1, Iva" . . "Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall"@en . "198450" . "10.1002/pro.744" . . . . . "Protein Science" . . "[657F5FD78C30]" . . "\u0160anda, Miloslav" . "Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall"@en . . . "P(GA310/09/1945), P(LC531), Z(AV0Z40550506)" . . "5"^^ . "Hru\u0161kov\u00E1, Olga" .