. "4"^^ . "Mac\u00ED\u010Dkov\u00E1-Cahov\u00E1, Hana" . . "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases"@en . . "Hocek, Michal" . . "alkynes; DNA; protecting groups; nucleotides; restriction endonucleases"@en . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "000295261200055" . "RIV/61388963:_____/11:00364215" . "The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs."@en . . . . "Kielkowski, Pavel" . "Angewandte Chemie. International Edition in English" . . "The first transient chemical protection of DNA against RE cleavage was developed. (Triethylsilyl)ethynyl-modified 7-deaza-dATP is readily incorporated to DNA by primer extension or PCR using KOD XL polymerase. The silylethynyl-protected DNA resists the cleavage by restriction endonucleases (REs). After treatment with NH3, the deprotected DNA is fully cleavable by the REs." . . "4"^^ . . "10.1002/anie.201102898" . "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases" . "50" . . . "Pohl, Radek" . "1433-7851" . "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases"@en . . "235826" . "37" . . . . . . . "P(GA203/09/0317), Z(AV0Z40550506)" . "[E710560DDEE4]" . . "RIV/61388963:_____/11:00364215!RIV12-AV0-61388963" . "Transient and Switchable (Triethylsilyl)ethynyl Protection of DNA against Cleavage by Restriction Endonucleases" . . . . "4"^^ .