"[C39437650D03]" . "Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases" . . . . "3" . "Pohl, Radek" . "RIV/61388963:_____/11:00360993" . "Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases"@en . "base-modified DNA; DNA cleavage; DNA polymerases; nucleosides; restriction endonucleases"@en . "Hocek, Michal" . . "Chembiochem" . "Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases" . "000287718700016" . "RIV/61388963:_____/11:00360993!RIV12-AV0-61388963" . . . . "P(GA203/09/0317), P(LC512), Z(AV0Z40550506)" . "A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved." . . "Mac\u00ED\u010Dkov\u00E1-Cahov\u00E1, Hana" . . "3"^^ . "10.1002/cbic.201000644" . . "A series of six pyrimidine modified dNTPs: 5-ethynyl- 5-phenyl- and 5-(3-nitrophenyl)-deoxycitidine and -deoxyuridine triphosphates were prepared and incorporated by primer extension using Vent (exo-) polymerase to specific DNA sequences within or next to the recognition sequences of selected restriction endonucleases. Then the cleavage of these pyrimidine-modified DNA sequences by 13 restriction enzymes was studied. While the presence of any modified C within the target sequence completely prevented any restriction cleavage, most enzymes tolerated the presence of 5-ethynylU and two of them even the presence of 5-phenyl- and 5-(3-nitrophenyl)U. Modifications outside the recognition sequence were tolerated apart from phenyl derivatives with PvuII enzyme. 5-EthynylC was used for protection of the recognition sequence from cleavage in presence of the second unmodified copy of the same sequence that was cleaved."@en . . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . . . "12" . . "3"^^ . . . . . . "8"^^ . "190552" . "Cleavage of Functionalized DNA Containing 5-Modified Pyrimidines by Type II Restriction Endonucleases"@en . "1439-4227" . . .