"Epigenetics" . . "Mertl\u00EDkov\u00E1-Kaiserov\u00E1, Helena" . . "6" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . "6"^^ . . "2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential"@en . "Matou\u0161ov\u00E1, Marika" . . "Votruba, Ivan" . "2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential" . "epigenetic therapy; nucleoside analogs; DNA methylation; 2'-deoxy-5-azacytidine; 2'-deoxy-5,6-dihydro-5-azacytidine"@en . . . "6"^^ . "Otmar, Miroslav" . "A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2\u00B4-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2\u00B4-deoxy-5,6-dihydro-5-azacytidine and its \u03B1-anomer, and of a 2-pyrimidone ribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2\u00B4-deoxycytidine 5\u00B4-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2\u00B4-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2\u00B4-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation." . . "6" . "1559-2294" . . "2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential"@en . "RIV/61388963:_____/11:00360024" . "A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2\u00B4-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2\u00B4-deoxy-5,6-dihydro-5-azacytidine and its \u03B1-anomer, and of a 2-pyrimidone ribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2\u00B4-deoxycytidine 5\u00B4-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2\u00B4-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2\u00B4-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation."@en . "8"^^ . "243997" . . . . "2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential" . . . . . "[1D0FE13F483A]" . "000291148100013" . "Tlou\u0161\u0165ov\u00E1, Eva" . . . . "RIV/61388963:_____/11:00360024!RIV12-AV0-61388963" . "P(1M0508), Z(AV0Z40550506)" . . . . . "10.4161/epi.6.6.16215" . . "Matou\u0161ov\u00E1, Marika" . "G\u00FCnterov\u00E1, Jana" .