"16" . "Widmann, J." . "10"^^ . "Chocholou\u0161ov\u00E1, Jana" . "Conservation is often used to define essential sequences within RNA sites. However, conservation finds only invariant sequence elements that are necessary for function, rather than finding a set of sequence elements sufficient for function. Here we define a critical test of sufficiency: We embed a minimal, apparently sufficient motif for binding the amino acid tryptophan in a random-sequence background and ask whether we obtain functional molecules. After a negative result, we use a combination of three-dimensional structural modeling, selection, designed mutations, high-throughput sequencing, and bioinformatics to explore functional insufficiency. This reveals an essential unpaired G in a diverse structural context, varied sequence, and flexible distance from the invariant internal loop binding site identified previously. Addition of the new element yields a sufficient binding site by the insertion criterion, binding tryptophan in 22 out of 23 tries." . "Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA"@en . "R N A" . . "Illangasekare, M." . . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . "McDonald, D." . "Conservation is often used to define essential sequences within RNA sites. However, conservation finds only invariant sequence elements that are necessary for function, rather than finding a set of sequence elements sufficient for function. Here we define a critical test of sufficiency: We embed a minimal, apparently sufficient motif for binding the amino acid tryptophan in a random-sequence background and ask whether we obtain functional molecules. After a negative result, we use a combination of three-dimensional structural modeling, selection, designed mutations, high-throughput sequencing, and bioinformatics to explore functional insufficiency. This reveals an essential unpaired G in a diverse structural context, varied sequence, and flexible distance from the invariant internal loop binding site identified previously. Addition of the new element yields a sufficient binding site by the insertion criterion, binding tryptophan in 22 out of 23 tries."@en . . "SELEX; molecular modeling; aptamer; evolution; tryptophan"@en . "RIV/61388963:_____/10:00353565" . . . "Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA"@en . "1"^^ . . "1355-8382" . "275569" . . . . "000281846500006" . "Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA" . "Reeder, J." . . "Knight, R." . . "Nucleotides that are essential but not conserved; a sufficient L-tryptophan site in RNA" . . "10" . . . "10"^^ . . "P(ME09019), Z(AV0Z40550506)" . "Iyer, M." . "Majerfeld, I." . . "[CB1BA2DF086A]" . . "Malaiya, V." . . "RIV/61388963:_____/10:00353565!RIV11-MSM-61388963" . "Yarus, M." .