"Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells"@en . . . "Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells" . . "5"^^ . . "Hol\u00FD, Anton\u00EDn" . . "9-[2-(phosphonomethoxy)ethyl] guanine (PMEG) is a guanine acyclic nucleotide analog whose prodrugs are being investigated for chemotherapy of lymphomas. The objective of this study was to determine the requirements for caspase and CD95/Fas activation in PMEG-induced apoptosis and the influence of PMEG on cell cycle regulatory proteins in CCRF-CEM cells. As for cell cycle regulation, cyclin E1 was found to be up-regulated following PMEG treatment. Although an increase of caspase 3, 8 and 9 proteolytic activity was observed, neither pretreatment of the cells with cell-permeable caspase inhibitors nor blocking the death receptor with anti-Fas antibody did prevent apoptosis induced by PMEG. It was concluded that PMEG-induced apoptosis is caspase- and CD95/Fas-independent."@en . "5"^^ . . . . "acyclic nucleoside phosphonate; programmed cell death; CCRF-CEM cells; cell cycle"@en . . "Mertl\u00EDkov\u00E1-Kaiserov\u00E1, Helena" . "Anticancer Research" . "Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells"@en . . "9-[2-(phosphonomethoxy)ethyl] guanine (PMEG) is a guanine acyclic nucleotide analog whose prodrugs are being investigated for chemotherapy of lymphomas. The objective of this study was to determine the requirements for caspase and CD95/Fas activation in PMEG-induced apoptosis and the influence of PMEG on cell cycle regulatory proteins in CCRF-CEM cells. As for cell cycle regulation, cyclin E1 was found to be up-regulated following PMEG treatment. Although an increase of caspase 3, 8 and 9 proteolytic activity was observed, neither pretreatment of the cells with cell-permeable caspase inhibitors nor blocking the death receptor with anti-Fas antibody did prevent apoptosis induced by PMEG. It was concluded that PMEG-induced apoptosis is caspase- and CD95/Fas-independent." . . . "7" . . . . "8"^^ . "[181DCABFC0B6]" . "0250-7005" . . . "Matou\u0161ov\u00E1, Marika" . "Role of Caspases and CD95/Fas in the Apoptotic Effects of a Nucleotide Analog PMEG in CCRF-CEM Cells" . . . "P(1M0508), Z(AV0Z40550506)" . "000280796400043" . "GR - \u0158eck\u00E1 republika" . . . "RIV/61388963:_____/10:00346305!RIV11-MSM-61388963" . "30" . "RIV/61388963:_____/10:00346305" . "285683" . "H\u00E1jek, Miroslav" . "Votruba, Ivan" . "Matou\u0161ov\u00E1, Marika" .