. . "Mertl\u00EDkov\u00E1-Kaiserov\u00E1, Helena" . . . . "7"^^ . "NL - Nizozemsko" . . . "European Journal of Pharmacology" . . "RIV/61388963:_____/10:00346304!RIV11-MSM-61388963" . . "acyclic nucleoside phosphonates; PMEG; PMEDAP; telomere length; telomerase inhibition"@en . "000280627700002" . "Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP"@en . "0014-2999" . "H\u00E1jek, Miroslav" . "We have previously shown that PMEG diphosphate and PMEDAP diphosphate inhibit the enzymatic activity of human telomerase in a cell-free assay. Here, we investigated the ability of PMEG and PMEDAP to induce telomere shortening and telomerase inhibition in two T-cell leukemia cell lines. Both PMEDAP and PMEG induced telomere shortening in CCRF-CEM cells after 9 weeks of exposure while the effect of the compounds on telomere length in MOLT-4 cells was the opposite. No correlation between telomerase activity and telomere length was found. Both compounds also down-regulated the expression of hTERT and c-myc mRNA in both cell lines. In conclusion, PMEDAP and PMEG are able to modulate telomere length in leukemic cells and this effect is cell-type specific. It is neither due to telomerase inhibition nor impairment of hTERT expression and it is likely to be telomerase-independent."@en . . "[0BE43D9D56E7]" . . "Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP" . . "Votruba, Ivan" . . . "Cvilink, Viktor" . . "5"^^ . "P(1M0508), P(1QS400550501), Z(AV0Z40550506)" . . "5"^^ . "254690" . . . . "Hol\u00FD, Anton\u00EDn" . "We have previously shown that PMEG diphosphate and PMEDAP diphosphate inhibit the enzymatic activity of human telomerase in a cell-free assay. Here, we investigated the ability of PMEG and PMEDAP to induce telomere shortening and telomerase inhibition in two T-cell leukemia cell lines. Both PMEDAP and PMEG induced telomere shortening in CCRF-CEM cells after 9 weeks of exposure while the effect of the compounds on telomere length in MOLT-4 cells was the opposite. No correlation between telomerase activity and telomere length was found. Both compounds also down-regulated the expression of hTERT and c-myc mRNA in both cell lines. In conclusion, PMEDAP and PMEG are able to modulate telomere length in leukemic cells and this effect is cell-type specific. It is neither due to telomerase inhibition nor impairment of hTERT expression and it is likely to be telomerase-independent." . . "1" . . "RIV/61388963:_____/10:00346304" . . . "643" . "Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP" . . "Distinct modulation of telomere length in two T-lymphoblastic leukemia cell lines by cytotoxic nucleoside phosphonates PMEG and PMEDAP"@en .