"The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism."@en . . . . . . "Biochemistry" . "Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods" . "19" . "P(1M0508), P(LC512), Z(AV0Z40550506)" . "0006-2960" . "Klus\u00E1k, Vojt\u011Bch" . . "Lubkowski, J." . . "5"^^ . "RIV/61388963:_____/09:00326498!RIV10-MSM-61388963" . . "48" . "Rul\u00ED\u0161ek, Lubom\u00EDr" . "RIV/61388963:_____/09:00326498" . "7"^^ . "Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods"@en . . . . . "The peptide hydrolysis by GCPII was investigated in detail by carrying out the kinetic analysis of GCPII(E424A) using N-Ac-Asp-Glu as substrate. The crystal structure of GCPII(E424A) in complex with N-Ac-Asp-Glu was determined at 1.70 A resolution. The experimental data were then complemented by the QM/MM calculations which provided detailed information concerning the GCPII reaction mechanism." . "Ba\u0159inka, C." . . "Konvalinka, Jan" . . . "Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods"@en . "reaction mechanism; QM/MM calculations; glutamate carboxypeptidase II; X-ray crystallography; mutant activity"@en . "Reaction mechanism of glutamate carboxypeptidase II revealed by mutagenesis, X-ray crystallography and computational methods" . "13"^^ . "000266047400012" . . "Plechanovov\u00E1, Anna" . . "Ml\u010Dochov\u00E1, Petra" . . . . "[1888AAD5DBAC]" . . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . . . "338184" .