"Rovensk\u00E1, Miroslava" . "Extracellular part of human glutamate carboxypeptidase II was co-crystallized with the phosphapeptide analogs of the enzyme substrates folyl-gamma-glutamate, aspartyl-glutamate, and gamma-glutamyl-glutamate. The determination of the crystal structures allowed us to describe the S1 site of the enzyme in detail for the first time and the corresponding enzyme-ligand interactions, too. Biochemical experiments provided additional information about the influence of the nature of the fragment in the P1 position of the substrate on the hydrolysis rate."@en . "Slusher, B. S." . "3"^^ . "13"^^ . "Hlouchov\u00E1, Kl\u00E1ra" . "5" . "Hin, N." . . "RIV/61388963:_____/08:00306830" . "Structural basis of interactions between human glutamate carboxypeptidase II and its substrate analogs"@en . "376" . "prostate-specific membrane antigen; metallopeptidase; folate hydrolase; NAALADase; phosphapeptide"@en . . "P(1M0508), Z(AV0Z40550506), Z(MSM 113100001)" . "397761" . "Majer, P." . "Struktur\u00E1ln\u00ED podstata interakc\u00ED lidsk\u00E9 glutam\u00E1t karboxypeptidasy II a analog\u016F jej\u00EDch substr\u00E1t\u016F"@cs . . "Journal of Molecular Biology" . "RIV/61388963:_____/08:00306830!RIV08-AV0-61388963" . . "Extracellular part of human glutamate carboxypeptidase II was co-crystallized with the phosphapeptide analogs of the enzyme substrates folyl-gamma-glutamate, aspartyl-glutamate, and gamma-glutamyl-glutamate. The determination of the crystal structures allowed us to describe the S1 site of the enzyme in detail for the first time and the corresponding enzyme-ligand interactions, too. Biochemical experiments provided additional information about the influence of the nature of the fragment in the P1 position of the substrate on the hydrolysis rate." . "Structural basis of interactions between human glutamate carboxypeptidase II and its substrate analogs"@en . "Struktur\u00E1ln\u00ED podstata interakc\u00ED lidsk\u00E9 glutam\u00E1t karboxypeptidasy II a analog\u016F jej\u00EDch substr\u00E1t\u016F"@cs . "Lubkowski, J." . . "Dauter, M." . . "Konvalinka, Jan" . "Structural basis of interactions between human glutamate carboxypeptidase II and its substrate analogs" . . . . "Extracelul\u00E1rn\u00ED \u010D\u00E1st lidsk\u00E9 glutam\u00E1tkarboxypeptidasy II byla vykrystalizov\u00E1na v komplexu s fosfapeptidov\u00FDmi analogy jej\u00EDch substr\u00E1t\u016F folyl-gamma-glutam\u00E1tu, aspartyl-glutam\u00E1tu a gamma-glutamyl-glutam\u00E1tu. Rentgenostrukturn\u00ED anal\u00FDza umo\u017Enila poprv\u00E9 detailn\u011B popsat S1 podm\u00EDsto enzymu a p\u0159\u00EDslu\u0161n\u00E9 interakce s ligandem. Dal\u0161\u00ED informace o vlivu druhu fragmentu v P1 pozici substr\u00E1tu na hydrol\u00FDzu poskytla dopl\u0148uj\u00EDc\u00ED biochemick\u00E1 m\u011B\u0159en\u00ED."@cs . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . . . "11"^^ . . . . . . "[D4BC3ACC3345]" . . "1438;1450" . "0022-2836" . . "Ba\u0159inka, C." . . "Ko, Y. S." . "Structural basis of interactions between human glutamate carboxypeptidase II and its substrate analogs" . "Tsukamoto, T." . . .