. "(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer" . . . "XXXII. Modern\u00ED elektrochemick\u00E9 metody. Sborn\u00EDk p\u0159edn\u00E1\u0161ek" . . . "3"^^ . . . . . . "Yosypchuk, O." . . "I, P(GAP206/11/1638), P(GAP208/12/1645), P(IAA400400806), S, Z(MSM0021620857)" . . "2"^^ . . "Jet\u0159ichovice" . "Mare\u010Dek, Vladim\u00EDr" . "voltammetry; amalgam electrodes; monolayer"@en . . . "2012-05-21+02:00"^^ . "4"^^ . . "RIV/61388955:_____/12:00377444!RIV13-GA0-61388955" . "BEST servis" . "978-80-905221-0-7" . "Yosypchuk, Bogdan" . . . . . "RIV/61388955:_____/12:00377444" . "(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer"@en . "\u00DAst\u00ED nad Labem" . "Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 \u03BCg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor." . "(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer" . "[4C41DC4370AF]" . . "Carboxylic group of 11-mercaptoundecanoic acid (MUA) can be used to creat a peptide bond with species containing amino group, e.g., peptides, and proteins. By the help of EDC-NHS technology, streptavidin or avidin was covalently bonded with MUA-monolayer at a silver solid amalgam electrode. Such prepared electrode was used for detecting biotin and biotinylated albumin in the supporting electrolyte (0.15 M NaCl, 0.05 M TRIS, pH 7.0). Electrochemical impendance spectroscopy was performed for the biosensor response monitored by impedance spectroscopy. Binding of biotin or biotinylated albumin with (strept)avidin a change in the resistance of the sensor in the concentration range of 0.5-20 \u03BCg mL-1. Electrochemical regeneration of the amalgam electrode permits simply to renew its surface and to create the new biosensor."@en . "(Strept)avidin-biotin interactions at amalgam electrodes covered by thiol monolayer"@en . "171681" . . "Yosypchuk, Bogdan" . .