. "Fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektrometrie" . "3"^^ . . "M\u00EDt mo\u017Enost sledovat proteiny nebo nukleov\u00E9 kyseliny na \u00FArovni jednotliv\u00FDch molekul je p\u0159\u00E1n\u00EDm t\u011Bch, kdo se sna\u017E\u00ED objasnit kl\u00ED\u010Dov\u00E9 procesy v \u017Eiv\u00FDch bu\u0148k\u00E1ch. Jednou z technik, kter\u00E1 se sna\u017E\u00ED toto p\u0159\u00E1n\u00ED naplnit, je fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopie (FCS). Metoda vznikla v 70. letech 20. stolet\u00ED. Od t\u00E9 doby se poda\u0159ilo v\u00FDrazn\u011B zlep\u0161it jej\u00ED experiment\u00E1ln\u00ED uspo\u0159\u00E1d\u00E1n\u00ED a byla vyu\u017Eita v \u0161irok\u00E9m spektru biologick\u00FDch aplikac\u00ED v\u010Detn\u011B m\u011B\u0159en\u00ED uvnit\u0159 \u017Eiv\u00FDch bun\u011Bk." . . "[7B4B86623FC8]" . . "Fluorescence Correlation Spectroscopy"@en . "5"^^ . . . "RIV/61388955:_____/09:00333812" . "103" . "Fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektrometrie" . . "000263745600002" . . . "Hof, Martin" . "2" . "Chemick\u00E9 listy" . "Fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektrometrie"@cs . . . "fluorescence correlation microscopy; autocorrelation function"@en . . "Basic principles of fluorescence correlation spectroscopy (FCS) are explained. The method affords diffusion coefficients and concentrations of fluorescent-labeled species by studying temporary fluctuations of fluorescence signal caused by diffusion through the focal volume of confocal microscope. In dual-color FCS, cross-correlation of two signals from molecules labeled with two spectrally shifted dyes allows to observe interactions between the two molecules. It is also possible to distinguish between the signals of two dyes with different lifetimes. FCS uses pulsed laser excitation."@en . "RIV/61388955:_____/09:00333812!RIV10-AV0-61388955" . "CZ - \u010Cesk\u00E1 republika" . "Z(AV0Z40400503)" . "Beranov\u00E1, Lenka" . "Humpol\u00ED\u010Dkov\u00E1, Jana" . . "0009-2770" . "Fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektrometrie"@cs . "315294" . "Fluorescence Correlation Spectroscopy"@en . "3"^^ . . . "M\u00EDt mo\u017Enost sledovat proteiny nebo nukleov\u00E9 kyseliny na \u00FArovni jednotliv\u00FDch molekul je p\u0159\u00E1n\u00EDm t\u011Bch, kdo se sna\u017E\u00ED objasnit kl\u00ED\u010Dov\u00E9 procesy v \u017Eiv\u00FDch bu\u0148k\u00E1ch. Jednou z technik, kter\u00E1 se sna\u017E\u00ED toto p\u0159\u00E1n\u00ED naplnit, je fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopie (FCS). Metoda vznikla v 70. letech 20. stolet\u00ED. Od t\u00E9 doby se poda\u0159ilo v\u00FDrazn\u011B zlep\u0161it jej\u00ED experiment\u00E1ln\u00ED uspo\u0159\u00E1d\u00E1n\u00ED a byla vyu\u017Eita v \u0161irok\u00E9m spektru biologick\u00FDch aplikac\u00ED v\u010Detn\u011B m\u011B\u0159en\u00ED uvnit\u0159 \u017Eiv\u00FDch bun\u011Bk."@cs . .