"GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "The present study has two main objectives. The first is to characterize antimicrobial peptide (AMP) cryptdin-4 (Crp-4) interactions with biological membranes and to compare those interactions with those of magainin 2. The second is to combine the complementary experimental approaches of laser scanning microscopy (LSM), ellipsometry, and Z-scan fluorescence correlation spectroscopy (FCS) to acquire comprehensive information on mechanisms of AMP interactions with supported phospholipid bilayers (SPBs) - a popular model of biological membranes. LSM shows appearance of inhomogencities in spatial distribution of lipids in the bilayer after treatment with Crp-4. Ellipsometric measurements show that binding of Crp-4 does not significantly change the lipid structure of the bilayer (increase in adsorbed mass without a change in thickness of adsorbed layer). Furthermore, Crp-4 slows the lateral diffusion of lipids within the membrane as shown by Z-scan FCS." . "Benda, Ale\u0161" . . "6"^^ . "1075-2617" . . "Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers" . . . . "Ouellette, A. J." . "cryptdin-4; megainin 2; supported phospholipid bilayers; ellipsometry"@en . . "14" . . "Hof, Martin" . "P(GA203/05/2308), P(GD203/05/H001), P(LC06063), Z(AV0Z40400503)" . "Mach\u00E1\u0148, Radek" . "Prezentovan\u00E1 studie m\u00E1 dva hlavn\u00ED c\u00EDle. Prvn\u00ED je charakterizovat interakce antimikrobi\u00E1ln\u00EDho peptidu (AMP) cryptdinu-4 (Crp-4) s biologick\u00FDmi membr\u00E1nami, a porovnat tyto interakce s interakcemi magaininu 2. Druh\u00FD je zkombinovat dopl\u0148kov\u00E9 experiment\u00E1ln\u00ED p\u0159\u00EDstupy laserov\u00E9 skenovac\u00ED mikroskopie (LSM), elipsometrie a z-skenov\u00E9 fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopie (FCS) pro z\u00EDsk\u00E1n\u00ED obs\u00E1hl\u00FDch informac\u00ED ohledn\u011B mechanismu AMP interakc\u00ED s podporovan\u00FDmi fosfolipidov\u00FDmi dvojvrstvami (SPBs) \u2013 popul\u00E1rn\u00EDm modelem biologick\u00FDch membr\u00E1n. LSM ukazuje vznik nehomogenit v prostorov\u00E9 distribuci lipidov\u00E9 dvojvrstvy po o\u0161et\u0159en\u00ED Crp-4. Elipsometrick\u00E1 m\u011B\u0159en\u00ED ukazuj\u00ED, \u017Ee vazba Crp-4 v\u00FDznamn\u011B nem\u011Bn\u00ED lipidovou strukturu dvojvrstvy (vzr\u016Fst adsorbovan\u00E9 hmoty bez zm\u011Bny tlou\u0161\u0165ky adsorbovan\u00E9 vrstvy). D\u00E1le Crp-4 zpomaluje later\u00E1ln\u00ED difusi lipid\u016F v membr\u00E1n\u011B, jak ukazuje Z-skenovac\u00ED FCS. V\u0161echny zm\u011Bny dvojvrstvy vyvolan\u00E9 Crp-4 mohou b\u00FDt \u010D\u00E1ste\u010Dn\u011B obr\u00E1ceny vymyt\u00EDm vzorku nadbytkem pufru."@cs . . "4" . . "Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers" . "Kombinace elipsometrie, laserov\u00E9 skenovac\u00ED mikroskopie a Z-skenov\u00E9 fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopie objas\u0148uje interakci cryptdinu-4 s podporovan\u00FDmi fosfolipidov\u00FDmi dvojvrstvami"@cs . "RIV/61388955:_____/08:00311018" . "Journal of Peptide Science" . "The present study has two main objectives. The first is to characterize antimicrobial peptide (AMP) cryptdin-4 (Crp-4) interactions with biological membranes and to compare those interactions with those of magainin 2. The second is to combine the complementary experimental approaches of laser scanning microscopy (LSM), ellipsometry, and Z-scan fluorescence correlation spectroscopy (FCS) to acquire comprehensive information on mechanisms of AMP interactions with supported phospholipid bilayers (SPBs) - a popular model of biological membranes. LSM shows appearance of inhomogencities in spatial distribution of lipids in the bilayer after treatment with Crp-4. Ellipsometric measurements show that binding of Crp-4 does not significantly change the lipid structure of the bilayer (increase in adsorbed mass without a change in thickness of adsorbed layer). Furthermore, Crp-4 slows the lateral diffusion of lipids within the membrane as shown by Z-scan FCS."@en . "Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers"@en . . "Kombinace elipsometrie, laserov\u00E9 skenovac\u00ED mikroskopie a Z-skenov\u00E9 fluorescen\u010Dn\u00ED korela\u010Dn\u00ED spektroskopie objas\u0148uje interakci cryptdinu-4 s podporovan\u00FDmi fosfolipidov\u00FDmi dvojvrstvami"@cs . . "Hermens, W. Th." . . "[AB476E9703CA]" . . "Combination of ellipsometry, laser scanning microscopy and Z-scan fluorescence correlation spectroscopy elucidating interaction of cryptdin-4 with supported phospholipid bilayers"@en . . "7"^^ . . . "4"^^ . . "000254901300022" . . "Miszta, Adam" . . . "Miszta, Adam" . . . "RIV/61388955:_____/08:00311018!RIV09-AV0-61388955" . "360392" .