. . "Yosypchuk, Bogdan" . . . "4"^^ . . . "Navr\u00E1til, Tom\u00E1\u0161" . "Jet\u0159ichovice" . "P\u0159\u00EDsp\u011Bvek se zab\u00FDv\u00E1 nov\u011B zkonstruovan\u00FDm 8-kan\u00E1lov\u00FDm p\u0159\u00EDstrojem, kter\u00FD umo\u017E\u0148uje sekven\u010Dn\u00ED anal\u00FDzu a\u017E na 8 r\u016Fzn\u00FDch nebo stejn\u00FDch \u010Didlech. P\u0159\u00EDstroj je \u0159\u00EDzen\u00FD po\u010D\u00EDta\u010Dem. K tomuto p\u0159\u00EDstroji byly p\u0159ipojov\u00E1ny uhl\u00EDkov\u00E9 ti\u0161t\u011Bn\u00E9 elektrody (SPCE). Byla detekov\u00E1na hybridizace hybridizace DNA. DNA p\u0159\u00EDtomn\u00E1 ve vzorku spont\u00E1nn\u011B adsorbovala na povrch SPCE a voln\u00FD povrch byl n\u00E1sledn\u011B blokov\u00E1n pomoc\u00ED hov\u011Bz\u00EDho s\u00E9rov\u00E9ho albuminu. Na adsorbovanou DNA byla pot\u00E9 hybridizov\u00E1na sonda zna\u010Den\u00E1 biotinem a na ni pak byl nav\u00E1z\u00E1n konjug\u00E1t streptavidinu s enzymem alkalickou fosfat\u00E1zou (SALP). Tento enzym katalyzuje p\u0159em\u011Bnu elektrochemicky neaktivn\u00EDho 1-naftylfosf\u00E1tu p\u0159\u00EDtomn\u00E9ho v elektrolytu na aktivn\u00ED 1-naftol, jeho\u017E anodick\u00FD sign\u00E1l byl detekov\u00E1n pomoc\u00ED stejnosm\u011Brn\u00E9 voltametrie (DCV)."@cs . "2007-05-21+02:00"^^ . "Fojta, Miroslav" . "The use of prototype of multichannel device for electrochemical detection of DNA hybridization"@en . "RIV/61388955:_____/07:00082672!RIV08-MSM-61388955" . "Praha" . . . . "Yosypchuk, Bogdan" . "The use of prototype of multichannel device for electrochemical detection of DNA hybridization"@en . "Br\u00E1zdilov\u00E1, P." . . . "P\u0159\u00EDsp\u011Bvek se zab\u00FDv\u00E1 nov\u011B zkonstruovan\u00FDm 8-kan\u00E1lov\u00FDm p\u0159\u00EDstrojem, kter\u00FD umo\u017E\u0148uje sekven\u010Dn\u00ED anal\u00FDzu a\u017E na 8 r\u016Fzn\u00FDch nebo stejn\u00FDch \u010Didlech. P\u0159\u00EDstroj je \u0159\u00EDzen\u00FD po\u010D\u00EDta\u010Dem. K tomuto p\u0159\u00EDstroji byly p\u0159ipojov\u00E1ny uhl\u00EDkov\u00E9 ti\u0161t\u011Bn\u00E9 elektrody (SPCE). Byla detekov\u00E1na hybridizace hybridizace DNA. DNA p\u0159\u00EDtomn\u00E1 ve vzorku spont\u00E1nn\u011B adsorbovala na povrch SPCE a voln\u00FD povrch byl n\u00E1sledn\u011B blokov\u00E1n pomoc\u00ED hov\u011Bz\u00EDho s\u00E9rov\u00E9ho albuminu. Na adsorbovanou DNA byla pot\u00E9 hybridizov\u00E1na sonda zna\u010Den\u00E1 biotinem a na ni pak byl nav\u00E1z\u00E1n konjug\u00E1t streptavidinu s enzymem alkalickou fosfat\u00E1zou (SALP). Tento enzym katalyzuje p\u0159em\u011Bnu elektrochemicky neaktivn\u00EDho 1-naftylfosf\u00E1tu p\u0159\u00EDtomn\u00E9ho v elektrolytu na aktivn\u00ED 1-naftol, jeho\u017E anodick\u00FD sign\u00E1l byl detekov\u00E1n pomoc\u00ED stejnosm\u011Brn\u00E9 voltametrie (DCV)." . . "4;7" . . "Vyu\u017Eit\u00ED prototypu multikan\u00E1lov\u00E9ho p\u0159\u00EDstroje pro elektrochemickou detekci hybridizace DNA" . . "460267" . "P(1H-PK/42), P(GA203/07/1195), P(IAA4004402), P(LC06035), Z(AV0Z40400503), Z(AV0Z50040702)" . . "\u010Cesk\u00E1 spole\u010Dnost chemick\u00E1" . . "978-80-86238-05-0" . "Sborn\u00EDk p\u0159edn\u00E1\u0161ek z XXVII. mezin\u00E1rodn\u00EDho odborn\u00E9ho semin\u00E1\u0159e" . "[0915F56D6D44]" . "Vyu\u017Eit\u00ED prototypu multikan\u00E1lov\u00E9ho p\u0159\u00EDstroje pro elektrochemickou detekci hybridizace DNA"@cs . "Vyu\u017Eit\u00ED prototypu multikan\u00E1lov\u00E9ho p\u0159\u00EDstroje pro elektrochemickou detekci hybridizace DNA" . "electrochemical detection; DNA"@en . "2"^^ . . "4"^^ . "The article presents newly constructed 8-channel device, which enables sequential analysis realized on up to 8 different or equal sensors. The device is computer controlled. The screen-printed carbon electrodes (SPCE) were connected to the device. DNA hybridization was detected. DNA present in the sample spontaneously adsorbed on the surface of SPCE and free surface was subsequently blocked using beef serum albumin. The adsorbed DNA was then hybridized with the probe marked with biotin and streptavidin conjugated with enzyme alkaline phosphatase (SALP). This enzyme catalyzes the transformation of electrochemically inactive 1-naphtylphosphateone 1-naphtol, which anodic signal was detected DC voltammetry (DCV)."@en . "RIV/61388955:_____/07:00082672" . . "Vyu\u017Eit\u00ED prototypu multikan\u00E1lov\u00E9ho p\u0159\u00EDstroje pro elektrochemickou detekci hybridizace DNA"@cs . . . .