. "2"^^ . "Voltametrick\u00E9 metody ve v\u00FDzkumu metalothionein\u016F"@cs . . . "Navr\u00E1til, Tom\u00E1\u0161" . . "549740" . "2003-04-01+02:00"^^ . "2"^^ . "43;53" . "Voltammetric Methods in Metallothionein Research"@en . "\u0160est\u00E1kov\u00E1, Ivana" . "Freund Publishing House Ltd" . "P(GA525/02/0301), P(OC D21.002), Z(AV0Z40400503)" . "Voltammetric Methods in Metallothionein Research" . "RIV/61388955:_____/05:00000692!RIV06-AV0-61388955" . "Guanajuato" . "Voltammetric Methods in Metallothionein Research" . . . "The application of voltammetric methods using different rates of polarisation on HMDE reveal inert or labile behaviour of Cd- or Zn- complexes in the presence of excessive cadmium or zinc ions in solution. This phenomenon was demonstrated first on the simplest phytochelatin - complex of peptide (.gamma.-Glu-Cys)2Gly with cadmium, later on rabbit liver metallothioneins - Cd7MT in the presence of cadmium and Cd5Zn2MT in the presence of zinc. Voltammetric methods can distinguish between labile and inert complexes present simultaneously and therefore could elucidate their role in reactions of metal ion transfer. Another method using different rates of polarisation - elimination voltammetry with linear scan - proved that S-tetracoordinated complexes of Cd(II) or Zn(II) in the above-mentioned metallothioneins on HMDE are reduced in the adsorbed state. This implies the possibility of increasing the sensitivity of identification or determination of the above complexes." . . . . "The application of voltammetric methods using different rates of polarisation on HMDE reveal inert or labile behaviour of Cd- or Zn- complexes in the presence of excessive cadmium or zinc ions in solution. This phenomenon was demonstrated first on the simplest phytochelatin - complex of peptide (.gamma.-Glu-Cys)2Gly with cadmium, later on rabbit liver metallothioneins - Cd7MT in the presence of cadmium and Cd5Zn2MT in the presence of zinc. Voltammetric methods can distinguish between labile and inert complexes present simultaneously and therefore could elucidate their role in reactions of metal ion transfer. Another method using different rates of polarisation - elimination voltammetry with linear scan - proved that S-tetracoordinated complexes of Cd(II) or Zn(II) in the above-mentioned metallothioneins on HMDE are reduced in the adsorbed state. This implies the possibility of increasing the sensitivity of identification or determination of the above complexes."@en . "RIV/61388955:_____/05:00000692" . "metallothioneins; phytochelatines; voltammetry; EVLS"@en . "Voltametrick\u00E9 metody ve v\u00FDzkumu metalothionein\u016F"@cs . "[70C808096460]" . . . "11"^^ . . . . "Aplikace voltametrick\u00FDch metod pou\u017E\u00EDvaj\u00EDc\u00EDch r\u016Fzn\u00E9 rychlosti polarizace na HMDE uk\u00E1zala inertn\u00ED nebo labiln\u00ED chov\u00E1n\u00ED Cd a Zn komplex\u016F, pokud jsou v roztoku p\u0159\u00EDtomny s nadbyte\u010Dn\u00FDmi ionty kadmia nebo zinku. Tento jev byl nejprve demonstrov\u00E1n na komplexu nejjednodu\u0161\u0161\u00EDho fytochelatinu (.gamma.-Glu-Cys)2Gly s kadmiem, d\u00E1le na metalothioneinech z kr\u00E1li\u010D\u00EDch jater \u2013 Cd7MT v p\u0159\u00EDtomnosti iont\u016F kadmia a Cd5Zn2MT v p\u0159\u00EDtomnosti iont\u016F zinku. Voltametrick\u00E9 metody mohou rozli\u0161it sou\u010Dasn\u011B p\u0159\u00EDtomn\u00E9 inertn\u00ED a labiln\u00ED komplexy a t\u00EDm objas\u0148ovat jejich roli v reakc\u00EDch p\u0159enosu iont\u016F kov\u016F. Elimina\u010Dn\u00ED voltametrie s line\u00E1rn\u00EDm scanem \u2013 dal\u0161\u00ED metoda pou\u017E\u00EDvaj\u00EDc\u00ED r\u016Fzn\u00E9 rychlosti polarizace \u2013 uk\u00E1zala, \u017Ee redukce S-tetrakoordinovan\u00FDch komplex\u016F Cd(II) \u010Di Zn (II) ve studovan\u00FDch metalothioneinech prob\u00EDh\u00E1 na HMDE v adsorbovan\u00E9m stavu. Z toho plyne mo\u017Enost zv\u00FD\u0161en\u00ED citlivosti p\u0159i identifikaci a stanoven\u00ED zm\u00EDn\u011Bn\u00FDch komplex\u016F."@cs . "Voltammetric Methods in Metallothionein Research"@en . . . . "1565-3633" . . . . . "Bioinorganic Chemistry and Applications" .