"4"^^ . . "A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals." . "107;110" . "-" . "RIV/61388955:_____/04:00101099!RIV/2005/AV0/A54005/N" . "Analytica Chimica Acta" . . . . "Opekar, F." . . . "0003-2670" . "Pro anal\u00FDzu 8-hydroxy-2\u00B4-deoxyguanosinu (8-OHdG) v mo\u010Di ob\u00E9zn\u00EDch pacient\u016F metodou HPLC byl testov\u00E1n jednoduch\u00FD amperometrick\u00FD detektor. Analyt byl extrahov\u00E1n z mo\u010Di s pou\u017Eit\u00EDm jednostup\u0148ov\u00E9 extrakce na pevn\u00E9 f\u00E1zi s pr\u016Fm\u011Brn\u00FDm v\u00FDt\u011B\u017Ekem 71%. HPLC byla prov\u00E1d\u011Bna na C-18 analytick\u00FDch kolon\u00E1ch za izokratick\u00FDch podm\u00EDnek. Zji\u0161t\u011Bn\u00FD limit detekce 8-OHdG (3nM) byl srovnateln\u00FD s limitem dosa\u017Een\u00FDm p\u0159i pou\u017Eit\u00ED komer\u010Dn\u011B dostupn\u00E9ho amperometrick\u00E9ho detektoru. Postup m\u011B\u0159en\u00ED byl pak pou\u017Eit pro ur\u010Den\u00ED hladin 8-OHdG v mo\u010Di 10 ob\u00E9zn\u00EDch pacient\u016F s v\u00FDsledkem 32+/-27,1 nM, kter\u00FD se p\u0159\u00EDli\u0161 neli\u0161il od hodnoty zji\u0161t\u011Bn\u00E9 u zdrav\u00FDch jedinc\u016F."@cs . "8-hydroxy-2''''-deoxyguanosine; DNA; obese patient"@en . "Samcov\u00E1, E." . "RIV/61388955:_____/04:00101099" . . . "Anal\u00FDza 8-hydroxy-2\u00B4-deoxyguanosinu v mo\u010Di ob\u00E9zn\u00EDch pacient\u016F metodou HPLC s elektrochemickou detekc\u00ED"@cs . "[FC0E0D1541D4]" . . "Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection" . . . . . "560141" . "Anal\u00FDza 8-hydroxy-2\u00B4-deoxyguanosinu v mo\u010Di ob\u00E9zn\u00EDch pacient\u016F metodou HPLC s elektrochemickou detekc\u00ED"@cs . . "Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection"@en . "NL - Nizozemsko" . "4"^^ . "Langmaier, Jan" . . "516" . . . "A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2''-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71 +/- 7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N = 3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index > 30 kg m(-2)) yielding the mean concentration 32 +/- 27.1 nM of 8-OHdG or 1.85 +/- 0.72 .mu.mol 8-OHdG mol(-1) creatinine (4.63 +/- 1.8. mu.g 8-OHdG g(-1) creatinine), which is not much different from the levels found in healthy individuals."@en . "Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection" . "Marhol, P." . "1"^^ . "P(GA203/01/0653), Z(AV0Z4040901)" . "Determination of Urinary 8-hydroxy-2''-deoxyguanosine in Obese Patiens by HPLC with Electrochemical Detection"@en .