. "RIV/60461373:22340/11:43892071!RIV12-MSM-22340___" . . "2011-10-24+02:00"^^ . . "22340" . "58. Konference chemick\u00E9ho a procesn\u00EDho in\u017Een\u00FDrstv\u00ED CHISA 2011" . . . . "190026" . . . "adhesion; microparticle; alginate; Silica"@en . "P\u0159edlo\u017Een\u00E1 pr\u00E1ce se zab\u00FDv\u00E1 p\u0159\u00EDpravou a charakterizac\u00ED algin\u00E1tov\u00FDch mikro\u010D\u00E1stic obalen\u00FDch vrstvou SiO2 pro c\u00EDlen\u00E9 vylu\u010Dov\u00E1n\u00ED l\u00E1tek. Kl\u00ED\u010Dov\u00FDm parametrem pro \u00FAsp\u011B\u0161nou aplikaci mikro\u010D\u00E1stic je vyhodnocen\u00ED jejich schopnosti adheze a to jak mezi sebou, tak i s \u017Eiv\u00FDmi bu\u0148kami nebo s povrchy substr\u00E1t\u016F. Volbou vhodn\u00E9 metody p\u0159\u00EDpravy a funkcionalizac\u00ED povrchu t\u011Bchto \u010D\u00E1stic je mo\u017En\u00E9 nasm\u011Brovat je na po\u017Eadovan\u00E9 c\u00EDle. Algin\u00E1tov\u00E9 mikro\u010D\u00E1stice o velikosti 40-80 ?m byly p\u0159ipraveny technologi\u00ED InkJet Drop on demand. Jejich povrch byl n\u00E1sledn\u011B upraven sol-gel procesem, p\u0159i kter\u00E9m do\u0161lo k vysr\u00E1\u017Een\u00ED SiO2 na povrchu algin\u00E1tu. Mikro\u010D\u00E1stice byly detailn\u011B charakterizov\u00E1ny pomoc\u00ED obrazov\u00E9 anal\u00FDzy (optick\u00FD mikroskop, SEM, LSCM) a byly pops\u00E1ny jejich z\u00E1kladn\u00ED parametry: tvar, velikost, tlou\u0161\u0165ka a porozita vrstvy. Bylo zji\u0161t\u011Bno, \u017Ee vrstva SiO2 je v rozmez\u00ED \u0159\u00E1du jednotek ?m a je vysoce por\u00E9zn\u00ED a flexibiln\u00ED. V dal\u0161\u00ED \u010D\u00E1sti pr\u00E1ce jsme se zab\u00FDvali studiem adheze mikro\u010D\u00E1stic k bun\u011B\u010Dn\u00FDm substr\u00E1t\u016Fm. Jako modelov\u00FD p\u0159\u00EDpad byly pou\u017Eity mikro\u010D\u00E1stice Dynabeads(R) (magnetick\u00E9 mikro\u010D\u00E1stice s proteinem G na povrchu). Nejd\u0159\u00EDve byly testov\u00E1ny interakce mikro\u010D\u00E1stic se specifick\u00FDmi receptory (monoklon\u00E1ln\u00ED protil\u00E1tkou) a n\u00E1sledn\u011B byla sledov\u00E1na adheze mikro\u010D\u00E1stic k prostatick\u00FDm n\u00E1dorov\u00FDm bu\u0148k\u00E1m (LNCaP cell) za r\u016Fzn\u00FDch podm\u00EDnek. Pokryt\u00ED povrchu \u010D\u00E1stic protil\u00E1tkou bylo ov\u011B\u0159eno gelovou elektrofor\u00E9zou. Vazba \u010D\u00E1stic na bun\u011B\u010Dn\u00FD povrch byla vyhodnocena optick\u00FDm mikroskopem." . "Characterization of composite silica-alginate microparticles and cell adhesion studies"@en . . "Dohnal, Ji\u0159\u00ED" . "\u0160t\u011Bp\u00E1nek, Franti\u0161ek" . "Haufov\u00E1, Petra" . . "6"^^ . . . . "P\u0159edlo\u017Een\u00E1 pr\u00E1ce se zab\u00FDv\u00E1 p\u0159\u00EDpravou a charakterizac\u00ED algin\u00E1tov\u00FDch mikro\u010D\u00E1stic obalen\u00FDch vrstvou SiO2 pro c\u00EDlen\u00E9 vylu\u010Dov\u00E1n\u00ED l\u00E1tek. Kl\u00ED\u010Dov\u00FDm parametrem pro \u00FAsp\u011B\u0161nou aplikaci mikro\u010D\u00E1stic je vyhodnocen\u00ED jejich schopnosti adheze a to jak mezi sebou, tak i s \u017Eiv\u00FDmi bu\u0148kami nebo s povrchy substr\u00E1t\u016F. Volbou vhodn\u00E9 metody p\u0159\u00EDpravy a funkcionalizac\u00ED povrchu t\u011Bchto \u010D\u00E1stic je mo\u017En\u00E9 nasm\u011Brovat je na po\u017Eadovan\u00E9 c\u00EDle. Algin\u00E1tov\u00E9 mikro\u010D\u00E1stice o velikosti 40-80 ?m byly p\u0159ipraveny technologi\u00ED InkJet Drop on demand. Jejich povrch byl n\u00E1sledn\u011B upraven sol-gel procesem, p\u0159i kter\u00E9m do\u0161lo k vysr\u00E1\u017Een\u00ED SiO2 na povrchu algin\u00E1tu. Mikro\u010D\u00E1stice byly detailn\u011B charakterizov\u00E1ny pomoc\u00ED obrazov\u00E9 anal\u00FDzy (optick\u00FD mikroskop, SEM, LSCM) a byly pops\u00E1ny jejich z\u00E1kladn\u00ED parametry: tvar, velikost, tlou\u0161\u0165ka a porozita vrstvy. Bylo zji\u0161t\u011Bno, \u017Ee vrstva SiO2 je v rozmez\u00ED \u0159\u00E1du jednotek ?m a je vysoce por\u00E9zn\u00ED a flexibiln\u00ED. V dal\u0161\u00ED \u010D\u00E1sti pr\u00E1ce jsme se zab\u00FDvali studiem adheze mikro\u010D\u00E1stic k bun\u011B\u010Dn\u00FDm substr\u00E1t\u016Fm. Jako modelov\u00FD p\u0159\u00EDpad byly pou\u017Eity mikro\u010D\u00E1stice Dynabeads(R) (magnetick\u00E9 mikro\u010D\u00E1stice s proteinem G na povrchu). Nejd\u0159\u00EDve byly testov\u00E1ny interakce mikro\u010D\u00E1stic se specifick\u00FDmi receptory (monoklon\u00E1ln\u00ED protil\u00E1tkou) a n\u00E1sledn\u011B byla sledov\u00E1na adheze mikro\u010D\u00E1stic k prostatick\u00FDm n\u00E1dorov\u00FDm bu\u0148k\u00E1m (LNCaP cell) za r\u016Fzn\u00FDch podm\u00EDnek. Pokryt\u00ED povrchu \u010D\u00E1stic protil\u00E1tkou bylo ov\u011B\u0159eno gelovou elektrofor\u00E9zou. Vazba \u010D\u00E1stic na bun\u011B\u010Dn\u00FD povrch byla vyhodnocena optick\u00FDm mikroskopem."@cs . "Charakteristika kompozitn\u00EDch SiO2-algin\u00E1tov\u00FDch mikro\u010D\u00E1stic a studium adheze"@cs . . "\u010Cesk\u00E1 spole\u010Dnost chemick\u00E9ho in\u017Een\u00FDrstv\u00ED" . "[17B26B428DAB]" . "3"^^ . "Srn\u00ED" . "Praha" . . "3"^^ . . "Charakteristika kompozitn\u00EDch SiO2-algin\u00E1tov\u00FDch mikro\u010D\u00E1stic a studium adheze" . "Characterization of composite silica-alginate microparticles and cell adhesion studies"@en . . . . . "P(GD104/08/H055), S" . "Charakteristika kompozitn\u00EDch SiO2-algin\u00E1tov\u00FDch mikro\u010D\u00E1stic a studium adheze"@cs . . "Charakteristika kompozitn\u00EDch SiO2-algin\u00E1tov\u00FDch mikro\u010D\u00E1stic a studium adheze" . "We prepared and characterized silica-alginate microparticles for controlled release applications [1]. A key parameter for their successful application is their ability to adhere to specific substrate (e.g. cells, proteins). Appropriate surface functionalization of these particles can direct them to the desired targets. Alginate microparticles (40 - 80 ?m) were prepared by InkJet Drop-on-demand technology. Sodium alginate is a natural polymer widely used for the encapsulation due to its biocompatibility and simple gelation with divalent cations such as Ca2+, Ba2+, etc [2, 3]. To control the porosity and adhesion, the alginate microparticles were subsequently coated with an SiO2 layer. Microparticles were characterized in detail by image analysis (optical microscope, SEM, LSCM) and their basic parameters (shape, size, thickness of the layer) were described. In the second part of the work, we studied the adhesion of microparticles to cell substrates. Dynabeads (R), magnetic microparticles with protein G on the surface, were used. First the magnetic microparticles were modified with specific receptors (monoclonal antibody) and then the interactions of microparticles with prostatic cancer cells were monitored. The potential binding of particles to cells was evaluated by image analysis."@en . "978-80-905035-0-2" . "RIV/60461373:22340/11:43892071" .