. "P(GA525/07/0903), Z(MSM6046137305), Z(MSM6046137307)" . "1" . . . . "000276042800006" . . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . "293186" . . . . . "Trace elements distribution and species fractionation in the wheat (Triticum aestivum) plant" . . . "Chemical Speciation and Bioavailability" . . "0954-2299" . . . . "Pol\u00E1k, J." . . "4"^^ . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "Kopl\u00EDk, Richard" . "Trace elements distribution and species fractionation in the wheat (Triticum aestivum) plant"@en . . . "22" . "Mestek, Oto" . "The status of 13 trace elements' (both essential and toxic) was investigated in individual parts of the winter wheat plant (Triticum aestivum) taken during its whole cultivation period. The study includes the determination of total concentrations, portions soluble in 0.02 mol L-1 Tris-HCl buffer solution (pH = 7.5), and the fractionation of soluble species of elements by SEC and ICPyMS. Ligands of trace elements from a low-molecular weight SEC fraction were isolated by affinity chromatography and characterised by MALDI/MS analyses and by amino acids composition. Inhomogeneous accumulation of trace elements was found in the analysed plant tissues. The concentrations of elements are also affected by the maturity of the plants. The distribution of the soluble species of the elements between chromatographic fractions exhibited some regularity in all the samples. Substantial amounts of trace elements are located in a low-molecular weight fraction (<2 kDa). Only chromatograms of Zn (grain) and Cu (a" . "Kom\u00EDnkov\u00E1, Jana" . . "Trace elements distribution and species fractionation in the wheat (Triticum aestivum) plant" . "wheat; speciation; trace elements; MALDI/MS; ICP/MS"@en . "Trace elements distribution and species fractionation in the wheat (Triticum aestivum) plant"@en . "RIV/60461373:22340/10:00023257!RIV11-GA0-22340___" . "22340" . . "10"^^ . . "5"^^ . . "The status of 13 trace elements' (both essential and toxic) was investigated in individual parts of the winter wheat plant (Triticum aestivum) taken during its whole cultivation period. The study includes the determination of total concentrations, portions soluble in 0.02 mol L-1 Tris-HCl buffer solution (pH = 7.5), and the fractionation of soluble species of elements by SEC and ICPyMS. Ligands of trace elements from a low-molecular weight SEC fraction were isolated by affinity chromatography and characterised by MALDI/MS analyses and by amino acids composition. Inhomogeneous accumulation of trace elements was found in the analysed plant tissues. The concentrations of elements are also affected by the maturity of the plants. The distribution of the soluble species of the elements between chromatographic fractions exhibited some regularity in all the samples. Substantial amounts of trace elements are located in a low-molecular weight fraction (<2 kDa). Only chromatograms of Zn (grain) and Cu (a"@en . . "RIV/60461373:22340/10:00023257" . "[2120253449FB]" .