. "22340" . "Fractionation of species of Cd, Cu, Mo, Ni and Zn in extracts of rye seedlings (grown up in both normal and Cd2+-enriched medium) and rye flour were performed by SEC/ICPMS method. Majority of Cu, Zn and Ni in all samples is bound in a 1-2 kDa fraction. Mo in all samples occurs in a fraction of Mr of 3 kDa. During five days of cultivation in a solution of 30 mol l-1 Cd2+ the plants accumulated as much as 5 mg kg-1 of Cd, but its soluble portion represents only 12-15%. Prevailing portion of Cd complexes is involved in a fraction of 3 kDa, while the minor part occurs in a fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of extracts of all samples (i.e. those of Mr of 1-2 kDa) were refined by immobilised metal affinity chromatography. Isolated ligands of trace elements are peptides rich in dicarboxylic amino-acids."@en . "Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings" . "Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings"@en . "RIV/60461373:22340/09:00021878!RIV10-MSM-22340___" . "Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings"@en . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . . "346612" . . . "Kom\u00EDnkov\u00E1, Jana" . . . . "Pol\u00E1k, Jan" . "rye; speciation; trace elements; MALDI-MS; ICP-MS; IMAC"@en . . "1212-1800" . . . "CZ - \u010Cesk\u00E1 republika" . "000264402600005" . . . . "Mestek, Oto" . "RIV/60461373:22340/09:00021878" . . . . "[DA44F51589FB]" . . . "27" . "Z(MSM6046137305), Z(MSM6046137307)" . . "6"^^ . "1" . "Trace elements species fractionation in rye flour and rye (Secale cereale) seedlings" . . "11"^^ . "5"^^ . . "Kopl\u00EDk, Richard" . "Czech Journal of Food Science" . . "Fractionation of species of Cd, Cu, Mo, Ni and Zn in extracts of rye seedlings (grown up in both normal and Cd2+-enriched medium) and rye flour were performed by SEC/ICPMS method. Majority of Cu, Zn and Ni in all samples is bound in a 1-2 kDa fraction. Mo in all samples occurs in a fraction of Mr of 3 kDa. During five days of cultivation in a solution of 30 mol l-1 Cd2+ the plants accumulated as much as 5 mg kg-1 of Cd, but its soluble portion represents only 12-15%. Prevailing portion of Cd complexes is involved in a fraction of 3 kDa, while the minor part occurs in a fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of extracts of all samples (i.e. those of Mr of 1-2 kDa) were refined by immobilised metal affinity chromatography. Isolated ligands of trace elements are peptides rich in dicarboxylic amino-acids." . . "Kod\u00ED\u010Dek, Milan" .