"Isolation of ligands of trace metals by immobilized metal affinity chromatography" . "Anal.Lett." . "4"^^ . . "Kod\u00ED\u010Dek, Milan" . . "Mestek, Oto" . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . "373398" . . . . "[2F5A4DE8ADE4]" . . "000257524800014" . . "Isolation of ligands of trace elements from plants by affinity chromatography with a metal immobilised on iminodiacetate resin has been investigated. To simulate various types of bioligands the following compounds were tested: phytic acid, peptides containing Cys and peptides containing Asp. Optimal conditions allowing isolation of the peptides with good efficiency comprise the use of Ga3+ ion, sample adsorption at pH=4 and elution of the compounds by 0.3 mol=l NH3. Phytic acid was bound too tightly and was not eluted. The procedure was used for purification of extracts of rye flour. The purified sample is suitable for analysis by MALDI-MS." . . . "P(GA525/07/0903), Z(MSM6046137307)" . "5"^^ . "Kopl\u00EDk, Richard" . "41" . . "RIV/60461373:22340/08:00020675" . . "Isolation of ligands of trace metals by immobilized metal affinity chromatography"@en . "Isolation of ligands of trace elements from plants by affinity chromatography with a metal immobilised on iminodiacetate resin has been investigated. To simulate various types of bioligands the following compounds were tested: phytic acid, peptides containing Cys and peptides containing Asp. Optimal conditions allowing isolation of the peptides with good efficiency comprise the use of Ga3+ ion, sample adsorption at pH=4 and elution of the compounds by 0.3 mol=l NH3. Phytic acid was bound too tightly and was not eluted. The procedure was used for purification of extracts of rye flour. The purified sample is suitable for analysis by MALDI-MS."@en . . . "9"^^ . . "Isolation of ligands of trace metals by immobilized metal affinity chromatography" . . "Isolation of ligands of trace metals by immobilized metal affinity chromatography"@en . "RIV/60461373:22340/08:00020675!RIV09-MSM-22340___" . "8" . "Izolace ligand\u016F stopov\u00FDch kov\u016F pomoc\u00ED afinitn\u00ED chromatografie s imobilizovan\u00FDm iontem kovu"@cs . "Pol\u00E1k, Jan" . . "22340" . . "Izolace ligand\u016F stopov\u00FDch kov\u016F pomoc\u00ED afinitn\u00ED chromatografie s imobilizovan\u00FDm iontem kovu"@cs . "C\u00EDlem v\u00FDzkumu byla izolace stopov\u00FDch prvk\u016F z extrakt\u016F rostlin pomoc\u00ED afinitn\u00ED chromatografie s ionty kovu imobilizovan\u00FDmi na pomoc\u00ED imidodiacet\u00E1tov\u00FDch funk\u010Dn\u00EDch skupin. Pro simulov\u00E1n\u00ED chov\u00E1n\u00ED r\u016Fzn\u00FDch typ\u016F bioligand\u016F byly vybr\u00E1ny n\u00E1sleduj\u00EDc\u00ED slou\u010Deniny: fytov\u00E1 kyselina, peptidy obsahuj\u00EDc\u00ED Cys a peptidy obsahuj\u00EDc\u00ED Asp. Optim\u00E1ln\u00ED podm\u00EDnky umo\u017E\u0148uj\u00EDc\u00ED dobrou v\u00FDt\u011B\u017Enost p\u0159i izolaci peptid\u016F zahrnovaly: pou\u017E\u00EDti iont\u016F Ga3+, nan\u00E1\u0161en\u00ED vzorku p\u0159i pH =4 a eluci zachycen\u00FDch l\u00E1tek 0,3 mol/l NH3. Fytov\u00E1 kyselina byla v\u00E1z\u00E1na p\u0159\u00EDli\u0161 pevn\u011B a nedo\u0161lo k jej\u00ED eluci. procedura byla aplikov\u00E1na na extrakty \u017Eitn\u00E9 mouky a izolovan\u00E9 l\u00E1tky byly analyzov\u00E1ny hmotnostn\u00ED spektrometri\u00ED MALDI."@cs . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . . "0003-2719" . . "Affinity chromatography; Speciation; Trace elements"@en .