"Zima, Tom\u00E1\u0161" . . "Kom\u00EDnkov\u00E1, Jana" . "The on-line coupling of LC and ICP-MS was used for fractionation and detection of species of Cu, Fe, I, Se and Zn in human serum. It has been shown that anion exchange chromatography proved better separation capability (both intra- and inter- element) than size exclusion chromatography. The mobile phases for ion exchange chromatography consisted of Tris-HNO3 buffer and ammonium salt (nitrate, acetate or formate). Formate was found to be the best mobile phase counter ion, which enables good chromatographic separation and is acceptable for mass spectrometer too. The quantitative evaluation of elements' concentrations adherent to individual fractions was performed by peak's area normalisation method. The repeatability of results ranges from 3 to 15 % (depending on the element fraction level) and represents the main part of result uncertainty. Accuracy of Cu and Zn fractions determination was confirmed by comparison with isotope dilution technique." . "21" . "446147" . . "5"^^ . . . . "5-14" . . "Applied Organometallic Chemistry" . . . "Kopl\u00EDk, Richard" . . . "Quantification of trace element species in human serum fractions by liquid chromatography and inductively coupled plasma mass spectrometry"@en . "Kod\u00ED\u010Dek, Milan" . . "RIV/60461373:22340/07:00019318" . "Serum; Trace elements; Metalloproteins; Liquid chromatography; Inductively coupled plasma mass spectrometry"@en . "4"^^ . . "Z(MSM6046137305), Z(MSM6046137307)" . . "Quantification of trace element species in human serum fractions by liquid chromatography and inductively coupled plasma mass spectrometry" . . "The on-line coupling of LC and ICP-MS was used for fractionation and detection of species of Cu, Fe, I, Se and Zn in human serum. It has been shown that anion exchange chromatography proved better separation capability (both intra- and inter- element) than size exclusion chromatography. The mobile phases for ion exchange chromatography consisted of Tris-HNO3 buffer and ammonium salt (nitrate, acetate or formate). Formate was found to be the best mobile phase counter ion, which enables good chromatographic separation and is acceptable for mass spectrometer too. The quantitative evaluation of elements' concentrations adherent to individual fractions was performed by peak's area normalisation method. The repeatability of results ranges from 3 to 15 % (depending on the element fraction level) and represents the main part of result uncertainty. Accuracy of Cu and Zn fractions determination was confirmed by comparison with isotope dilution technique."@en . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "RIV/60461373:22340/07:00019318!RIV08-MSM-22340___" . . "0268-2605" . "Quantification of trace element species in human serum fractions by liquid chromatography and inductively coupled plasma mass spectrometry" . "[6FB76861EF3B]" . "22340" . . "Frakcionace speci\u00ED Cu, Fe, I, Se a Zn v lidsk\u00E9m s\u00E9ru byla provedena pomoc\u00ED on-line spojen\u00ED kapalinov\u00E9 chromatografie a pomoc\u00ED hmotnostn\u00ED spekrometrie s induk\u010Dn\u011B v\u00E1zan\u00FDm plazmatem. Iontov\u011B v\u00FDm\u011Bnn\u00E1 chromatografie se uk\u00E1zala jako metoda vhodn\u011Bj\u0161\u00ED ne\u017E gelov\u00E1 chromatografie. Jako nejvhodn\u011Bj\u0161\u00ED mobiln\u00ED f\u00E1ze byl pou\u017Eit mraven\u010Danov\u00FD pufr. Kvantitativn\u00ED vyhodnocen\u00ED obsahu kov\u016F v jednotliv\u00FDch bylo provedeno pomoc\u00ED normalizace p\u00EDk\u016F, opakovatelnost byla 3 - 15 %. Spr\u00E1vnost stanoven\u00ED Cu a Zn byla ov\u011B\u0159ena pomoc\u00ED izotopov\u00E9ho z\u0159e\u010Fov\u00E1n\u00ED."@cs . . . "Kvantifikace speci\u00ED stopov\u00FDch prvk\u016F v lidsk\u00E9m s\u00E9ru pomoc\u00ED kapalinov\u00E9 chromatografie a hmotnostn\u00ED spektrometrie s induk\u010Dn\u011B v\u00E1zan\u00FDm plazmatem"@cs . "1" . "Kvantifikace speci\u00ED stopov\u00FDch prvk\u016F v lidsk\u00E9m s\u00E9ru pomoc\u00ED kapalinov\u00E9 chromatografie a hmotnostn\u00ED spektrometrie s induk\u010Dn\u011B v\u00E1zan\u00FDm plazmatem"@cs . . . "Quantification of trace element species in human serum fractions by liquid chromatography and inductively coupled plasma mass spectrometry"@en . "10"^^ . . "Mestek, Oto" .