. . "[F7DEA8C9D35A]" . . . . "\u010Cesk\u00FD T\u011B\u0161\u00EDn" . . "3"^^ . . . "36-40" . "RIV/60461373:22340/07:00018400" . "Izolace n\u00EDzkomolekul\u00E1rn\u00EDch ligand\u016F stopov\u00FDch prvk\u016F z rostlinn\u00E9ho materi\u00E1lu pomoc\u00ED afinitn\u00ED chromatografie" . "Isolation of trace elements ligands from plant material by immobilized metal affinity chromatography"@en . . . "427715" . "Mestek, Oto" . . "2"^^ . "5"^^ . "P\u0159edkl\u00E1dan\u00E1 pr\u00E1ce je zam\u011B\u0159ena na optimalizaci afinitn\u00ED chromatografie s v\u00E1zan\u00FDm iontem kovu pro izolaci \u0159ady ligand\u016F stopov\u00FDch prvk\u016F zastoupen\u00FDch v rostlinn\u00FDch materi\u00E1lech pro jejich dal\u0161\u00ED strukturn\u00ED anal\u00FDzu.Testovan\u00FDmi ionty kovu byly: Ga3+, Cu2+, Ni2+, Co2+. pH reten\u010Dn\u00EDho pufru bylo upravov\u00E1no v rozmez\u00ED 7,5-2. Ligandy pou\u017Eit\u00E9 pro testov\u00E1n\u00ED byly peptidy s a bez p\u0159edpokl\u00E1dan\u00E9 afinity k iont\u016Fm kovu. Nejlep\u0161\u00EDch v\u00FDt\u011B\u017Ek\u016F izolace bylo dosa\u017Eeno p\u0159i pou\u017Eit\u00ED v\u00E1zan\u00FDch Ga3+ iont\u016F a pH reten\u010Dn\u00EDho pufru 4. Metoda vykazuje dobrou selektivitu pro vybran\u00E9 ligandy a byla pou\u017Eita pro izolaci p\u0159\u00EDrodn\u00EDch ligand\u016F z extraktu \u017Eitn\u00E9 mouky."@cs . "P\u0159edkl\u00E1dan\u00E1 pr\u00E1ce je zam\u011B\u0159ena na optimalizaci afinitn\u00ED chromatografie s v\u00E1zan\u00FDm iontem kovu pro izolaci \u0159ady ligand\u016F stopov\u00FDch prvk\u016F zastoupen\u00FDch v rostlinn\u00FDch materi\u00E1lech pro jejich dal\u0161\u00ED strukturn\u00ED anal\u00FDzu.Testovan\u00FDmi ionty kovu byly: Ga3+, Cu2+, Ni2+, Co2+. pH reten\u010Dn\u00EDho pufru bylo upravov\u00E1no v rozmez\u00ED 7,5-2. Ligandy pou\u017Eit\u00E9 pro testov\u00E1n\u00ED byly peptidy s a bez p\u0159edpokl\u00E1dan\u00E9 afinity k iont\u016Fm kovu. Nejlep\u0161\u00EDch v\u00FDt\u011B\u017Ek\u016F izolace bylo dosa\u017Eeno p\u0159i pou\u017Eit\u00ED v\u00E1zan\u00FDch Ga3+ iont\u016F a pH reten\u010Dn\u00EDho pufru 4. Metoda vykazuje dobrou selektivitu pro vybran\u00E9 ligandy a byla pou\u017Eita pro izolaci p\u0159\u00EDrodn\u00EDch ligand\u016F z extraktu \u017Eitn\u00E9 mouky." . "RIV/60461373:22340/07:00018400!RIV08-MSM-22340___" . . "Izolace n\u00EDzkomolekul\u00E1rn\u00EDch ligand\u016F stopov\u00FDch prvk\u016F z rostlinn\u00E9ho materi\u00E1lu pomoc\u00ED afinitn\u00ED chromatografie" . "Izolace n\u00EDzkomolekul\u00E1rn\u00EDch ligand\u016F stopov\u00FDch prvk\u016F z rostlinn\u00E9ho materi\u00E1lu pomoc\u00ED afinitn\u00ED chromatografie"@cs . "Mikroelementy 2007" . . "speciation; metal affinity chromatography; ICP-MS; IMAC"@en . . "Kopl\u00EDk, Richard" . . . "Kouty" . . "22340" . . "2THETA" . "2007-09-03+02:00"^^ . "Pol\u00E1k, Jan" . . . "Presenting work is focused on optimalization if Imobilized metal affinity chromatography which represents a powerfull method for isolation and purification of trace metal ligands occuring in plant materials. The refined sample is suitable for mass spectrometry analysis. ). Four different metal ions were utilized: Cu2+, Ni2+, Co2+ and Ga3+. The highest value of pH was 7.5 The pH of the buffer solutions was successively decreased down to a value of 2. Ligands used for testing were peptides with and without assumed affinity to metal ions. Optimum conditions allowing an isolation of peptides with good efficiency comprise the use of Ga3+ ion and sample adsorption at pH=4. Used method shows good selectivity for chosen ligands and was used for isolation natural ligands from rye flour extract."@en . . "Izolace n\u00EDzkomolekul\u00E1rn\u00EDch ligand\u016F stopov\u00FDch prvk\u016F z rostlinn\u00E9ho materi\u00E1lu pomoc\u00ED afinitn\u00ED chromatografie"@cs . "978-80-86380-40-7" . "P(GA525/07/0903), Z(MSM6046137305), Z(MSM6046137307)" . "Isolation of trace elements ligands from plant material by immobilized metal affinity chromatography"@en .