"Mestek, Oto" . . "Anal\u00FDza frakc\u00ED speci\u00ED prvk\u016F v pseudocere\u00E1li\u00EDch pomoc\u00ED SEC/ICP-MS a MALDI-MS"@cs . "Kom\u00EDnkov\u00E1, Jana" . . "Kopl\u00EDk, Richard" . "Analysis of elements species fractions in pseudo-cereals by SEC/ICP-MS and MALDI-MS"@en . . "Analysis of elements species fractions in pseudo-cereals by SEC/ICP-MS and MALDI-MS" . . . . . . "Analysis of elements species fractions in pseudo-cereals by SEC/ICP-MS and MALDI-MS"@en . "European Food Research and Technology" . . . . "RIV/60461373:22340/07:00018397!RIV08-MSM-22340___" . "Kvasni\u010Dka, Franti\u0161ek" . "Pol\u00E1k, Jan" . "1438-2377" . "22340" . . . "RIV/60461373:22340/07:00018397" . "225" . . "P(GA525/04/0503), Z(MSM6046137307)" . . "Analysis of elements species fractions in pseudo-cereals by SEC/ICP-MS and MALDI-MS" . "6"^^ . "7"^^ . . . . . "Anal\u00FDza frakc\u00ED speci\u00ED prvk\u016F v pseudocere\u00E1li\u00EDch pomoc\u00ED SEC/ICP-MS a MALDI-MS"@cs . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . . "Frakcionace speci\u00ED Co, Cu, Fe, Mn, Mo, Ni, P a Zn v extraktu pohankov\u00E9 krupice a amarantov\u00E9 mouky v pufru 0,02 molxl-1 Tris-HCl (pH=7,5) byla provedena pomoc\u00ED spojen\u00ED SEC/ICP-MS. Prvkov\u011B specifick\u00E9 chromatogramy extraktu pohanky jsou podobn\u00E9 lu\u0161t\u011Bnin\u00E1m a cere\u00E1li\u00EDm. S v\u00FDjimkou Fe a Mn jsou zbyl\u00E9 kovy obsa\u017Eeny v n\u00EDzkomolekul\u00E1rn\u00ED frakci (Mr<2 kDa). Fe je v\u00E1z\u00E1no ve vysoko molekul\u00E1rn\u00ED frakci (Mr>150 kDa). Chromatogramy speci\u00ED Co, Fe, Mo a Ni v extraktu amarantov\u00E9 mouky odpov\u00EDdaj\u00ED tak\u00E9 tomuto vzoru. Cu je v\u00E1z\u00E1na tak\u00E9 ve st\u0159edn\u011B molekul\u00E1rn\u00ED frakci (Mr~40 kDa) a vysoko molekul\u00E1rn\u00ED frakci (Mr>150 kDa). Hlavn\u00ED pod\u00EDl Mn v obou extraktech je p\u0159\u00EDtomen ve form\u011B labiln\u00EDch komlex\u016F nebo iont\u016F. Frakce obsahuj\u00EDc\u00ED vysok\u00E1 mno\u017Estv\u00ED kov\u016F byly izolov\u00E1ny pomoc\u00ED preparativn\u00ED SEC a organick\u00E9 ligandy kov\u016F obsa\u017Een\u00E9 v t\u011Bchto frakc\u00EDch byly n\u00E1sledn\u011B zakoncentrov\u00E1ny pomoc\u00ED Chelexu 100 v Cu2+ form\u011B. P\u0159e\u010Di\u0161t\u011Bn\u00E9 ligandy obsahovaly n\u00EDzk\u00E1 mno\u017Estv\u00ED sirn\u00FDch aminokyselin (Cys, Met) a vysok\u00E1 mno\u017Estv\u00ED kysel\u00FDch aminokyselin (Glu, Asp)"@cs . "409462" . . . . "Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 molxl-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in a labile complex or an ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and hig" . "[580D5F7039DF]" . "Kod\u00ED\u010Dek, Milan" . . "Amaranth; buckwheat; speciation; trace elements; MALDI-MS; ICP-MS"@en . "5-6" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . "10"^^ . . "895-904" . "Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 molxl-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in a labile complex or an ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and hig"@en .