"Interaction of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) with chitosan (Mr 400 kDa, N-acetyls 20 mol.%) was studied in aqueous solutions. UV-vis absorption and circular dichroism (CD) spectroscopic titration of 10 \u03BCmol l-1 TPPS4 with chitosan demonstrated that an addition of the polysaccharide at appropriate concentrations and pH values induce and support self-aggregation of the macrocycles. The mode of aggregation was strongly dependent on pH: stacking (H-type) aggregates predominated at weak acidic conditions (pH 4.8-6.8) and tilted (J-type) aggregates at pH 2.5. At the intermediate pH value (3.6) both types of TPPS4 aggregates were detected. High amount of chitosan (>0.05 mmol l-1 of GlcN) disrupts H-aggregates forming monomeric porphyrin-chitosan complexes (pH 3.6-6.8), while J-aggregates (pH 2.5) are stable even at very high chitosan concentrations. CD titration experiments confirmed the formation of optically active species of TPPS4 in the presence of chitosan. The complex nat"@en . "TPPS4; Chitosan; Aggregation; UV-vis absorption; Circular dichroism"@en . "Interaction of meso-tetrakis(4-sulphonatophenyl)porphine (TPPS4) with chitosan (Mr 400 kDa, N-acetyls 20 mol.%) was studied in aqueous solutions. UV-vis absorption and circular dichroism (CD) spectroscopic titration of 10 \u03BCmol l-1 TPPS4 with chitosan demonstrated that an addition of the polysaccharide at appropriate concentrations and pH values induce and support self-aggregation of the macrocycles. The mode of aggregation was strongly dependent on pH: stacking (H-type) aggregates predominated at weak acidic conditions (pH 4.8-6.8) and tilted (J-type) aggregates at pH 2.5. At the intermediate pH value (3.6) both types of TPPS4 aggregates were detected. High amount of chitosan (>0.05 mmol l-1 of GlcN) disrupts H-aggregates forming monomeric porphyrin-chitosan complexes (pH 3.6-6.8), while J-aggregates (pH 2.5) are stable even at very high chitosan concentrations. CD titration experiments confirmed the formation of optically active species of TPPS4 in the presence of chitosan. The complex nat" . "11"^^ . "66" . "Interaction of meso-tetrakis(4-sulphonatophenyl)porphine with chitosan in aqueous solutions" . "Volka, Karel" . . . "NL - Nizozemsko" . "2" . "Kr\u00E1l, Vladim\u00EDr" . "Interakce meso-tetrakis(4-sulphonatophenyl)porphinu s chitosanem ve vodn\u00EDm"@cs . "Synytsya, Andriy" . "Blafkov\u00E1, Petra" . . . . "22340" . "P(GA203/02/0420), Z(MSM6046137305), Z(MSM6046137307)" . "1386-1425" . "[0E886FE03517]" . "RIV/60461373:22340/07:00018392!RIV08-MSM-22340___" . "Interakce meso-tetrakis(4-sulphonatophenyl)porphinu s chitosanem (Mr 400 kDa, N-acetyls 20 mol.%) byla studovan\u00E1 ve vod\u00EDm prost\u0159ed\u00ED. Spektroskopick\u00E1 titrace (UV-vis absorp\u010Dn\u00ED a CD) 10 \u03BCmol l-1 TPPS4 s chitosanem prok\u00E1zala \u017Ee ur\u010Dit\u00E9 koncentrace roztoku polysacharidu podporuj\u00ED vzniknuti vysoce uspo\u0159\u00E1dan\u00FDch struktur makrociklu. Typ uspo\u0159\u00E1dan\u00ED je z\u00E1visl\u00FD na pH roztoku. Tak pH 2.5 podporuj\u00ED uspo\u0159\u00E1dan\u00ED podle typu - J, kdy\u017E slabo-kysele prost\u0159ed\u00ED (pH 4.8-6.8) podporuj\u00ED uspo\u0159\u00E1dan\u00ED podle typu - H. Oba typy uspo\u0159\u00E1dan\u00ED projevovali optickou aktivitu."@cs . . . . "427052" . . . . "Interakce meso-tetrakis(4-sulphonatophenyl)porphinu s chitosanem ve vodn\u00EDm"@cs . . . . . "4"^^ . "225-235" . . "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy" . "Synytsya, Alla" . "Interaction of meso-tetrakis(4-sulphonatophenyl)porphine with chitosan in aqueous solutions"@en . "Interaction of meso-tetrakis(4-sulphonatophenyl)porphine with chitosan in aqueous solutions" . . . . "RIV/60461373:22340/07:00018392" . "5"^^ . . . "Interaction of meso-tetrakis(4-sulphonatophenyl)porphine with chitosan in aqueous solutions"@en . . .