"P(GP104/03/D006), Z(MSM6046137306)" . . "HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS"@en . "RIV/60461373:22340/06:00016656" . "RIV/60461373:22340/06:00016656!RIV07-MSM-22340___" . "Pr\u00E1ce je zam\u011B\u0159ena na v\u00FDvoj mikrofluidn\u00EDch za\u0159\u00EDzen\u00ED pro kvantitativn\u00ED stanoven\u00ED IgG. Za\u0159\u00EDzen\u00ED je vyrobeno technikou mikroobr\u00E1b\u011Bn\u00ED. Experiment\u00E1ln\u00ED data dokazuj\u00ED, \u017Ee kvantitativn\u00ED detekce IgG v navr\u017Een\u00E9m multikan\u00E1lov\u00E9m mikro\u010Dipu je mo\u017En\u00E1 b\u011Bhem n\u011Bkolika minut."@cs . . . . "1"^^ . "Tatransk\u00E9 Matliare" . . "Marek, Milo\u0161" . . . "3"^^ . "[9C70AED74EFC]" . "The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few" . . "Heteogenn\u00ED imunoanal\u00FDza v polystyrenov\u00E9m mikro\u010Dipu"@cs . "The work is focused on development of microfluidic polystyrene chips for detection of biological agents. The microfluidic chips are constructed with the use of the micro-milling technology. The interaction among the immobilized bio-molecules and the complementary molecules from an analyte is the principle of the microfluidic chip. Here, the immobilized Protein A (PA) binds the complementary human immunoglobulin G (IgG) from the liquid sample and the protein-protein complex is formed. Because the immunoglobulins are fluorescently labeled, the observed fluorescence signal corresponds with the concentration of the biological macromolecule in the sample. Specificity of the interaction PA - human IgG is tested in blind experiments. Bovine serum albumin (BSA) is used as the blocking agent and Tris buffer with detergent Tween disintegrates the non-specific bonds. The experimental results demonstrate that the immunoglobulins can be quantitatively detected in the proposed multichannel microchip within few"@en . . . . "075(1-6)" . "22340" . . "P\u0159ibyl, Michal" . . "Slovak Society of Chemical Engineering" . "2006-05-22+02:00"^^ . . . "heterogeneous immunoassay; antigen; antibody; microchip; microdevice; microsensor; protein A; human IgG"@en . "80-227-2409-2" . "Bratislava" . . . . . . "477615" . "33rd International Conference of SSCHE, Tatransk\u00E9 Matliare" . "\u0160t\u011Bp\u00E1nek, Jakub" . "6"^^ . "HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS"@en . . . "HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS" . . "Heteogenn\u00ED imunoanal\u00FDza v polystyrenov\u00E9m mikro\u010Dipu"@cs . "HETEROGENEOUS IMMUNOASSAY IN POLYSTYRENE MICROCHIPS" .