"Tatransk\u00E9 Matliare, Slovensko" . "DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG"@en . "V\u00FDvoj experiment\u00E1ln\u00EDho mikroza\u0159\u00EDzen\u00ED pro detekci IgG"@cs . "Microfluidic devices are becoming an important tool in diagnostics of diseases and analysis of biological liquids. In this work, we present our progress in development of a microchip for ultra-fast and quantitative determination of human antibodies (IgG) in an aqueous sample. The method is based on the specific biochemical interaction between IgG and the immobilized antigen (Protein A). The antibody molecules are fluorescently labeled and thus their concentration in the microdevice can be monitored by fluorescence microscope. The microdevice consists of an active polystyrene part (PS) with a thin microcappilary and a part for connection of the PS part to the sample and the washing solutions. The microcapillary has diameter between 10 and 20 microns. Use of the electrokinetic phenomena (electroosmosis) for transportation of liquids in the PS part is intensively studied. All step of the immunoassay are described and the experimental determination of IgG concentration in a sample by means of the proposed" . . "[051C3A39BFBD]" . . "Proc. of 32th International Conference of Slovak Society of Chemical Engineering." . "\u0160nita, Dalimil" . "\u0160t\u011Bp\u00E1nek, Jakub" . "Bratislava" . "immunoassay; microchip; hot embossing; electroosmosis; fluorescence; molding"@en . . . "DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG" . . . "RIV/60461373:22340/05:00014595!RIV06-GA0-22340___" . "517744" . . "V\u00FDvoj experiment\u00E1ln\u00EDho mikroza\u0159\u00EDzen\u00ED pro detekci IgG"@cs . "Slovensk\u00E1 technick\u00E1 univerzita v Bratislave" . . "P\u0159ibyl, Michal" . "064/1-064/7" . . "80-227-2224-3" . . . "Microfluidic devices are becoming an important tool in diagnostics of diseases and analysis of biological liquids. In this work, we present our progress in development of a microchip for ultra-fast and quantitative determination of human antibodies (IgG) in an aqueous sample. The method is based on the specific biochemical interaction between IgG and the immobilized antigen (Protein A). The antibody molecules are fluorescently labeled and thus their concentration in the microdevice can be monitored by fluorescence microscope. The microdevice consists of an active polystyrene part (PS) with a thin microcappilary and a part for connection of the PS part to the sample and the washing solutions. The microcapillary has diameter between 10 and 20 microns. Use of the electrokinetic phenomena (electroosmosis) for transportation of liquids in the PS part is intensively studied. All step of the immunoassay are described and the experimental determination of IgG concentration in a sample by means of the proposed"@en . "P(GP104/03/D005), Z(MSM6046137306)" . "3"^^ . "7"^^ . . "DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG"@en . "DEVELOPMENT OF AN EXPERIMENTAL MICRODEVICE FOR DETECTION OF IGG" . . "RIV/60461373:22340/05:00014595" . . "22340" . "V t\u00E9to pr\u00E1ci je prezentov\u00E1n n\u00E1\u0161 pokrok ve v\u00FDvoji mikro\u010Dip\u016F pro ultrarychl\u00E9 a kvantitativn\u00ED stanoven\u00ED lidsk\u00FDch protil\u00E1tek ve vodn\u00E9m roztoku.Jsou prezentov\u00E1ny v\u0161echny kroky v\u00FDroby mikrofluidn\u00EDho za\u0159\u00EDzen\u00ED a jednotliv\u00E9 kroky imunoanal\u00FDzy. Jsou diskutov\u00E1ny mo\u017En\u00E9 v\u00FDhody I nev\u00FDhody pou\u017Eit\u00E9ho mikro\u010Dipu."@cs . "4"^^ . . . . . . . "2005-05-23+02:00"^^ . . . . "Lindner, Ji\u0159\u00ED" .