"Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis" . . "98" . . "11500" . . "Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species." . . "22330" . . "Kr\u00E1sn\u00FD, Luk\u00E1\u0161" . "Rohlov\u00E1, Eva" . . "RIV/60461373:22330/14:43897784" . "10.1016/j.mimet.2014.01.008" . "R\u016F\u017Ei\u010Dkov\u00E1, Helena" . "Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis"@en . "Journal of Microbiological Methods" . "Trypsin; Mass spectrometry; MALDI-TOF; Cronobacter; Biotyper"@en . "I, P(GAP503/10/0664), S" . "0167-7012" . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . . "Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis" . "Hochel, Igor" . . . . . . "http://ac.els-cdn.com/S0167701214000207/1-s2.0-S0167701214000207-main.pdf?_tid=0fe3df92-a861-11e4-8f66-00000aab0f6c&acdnat=1422609736_0d9b8721de703da1123ae5ee6262e32d" . "[584F7B2B94F2]" . . "6"^^ . "000333852700019" . . . "NL - Nizozemsko" . "Hynek, Radovan" . . . "RIV/60461373:22330/14:43897784!RIV15-GA0-22330___" . . "5"^^ . . . "Differentiation of Cronobacter spp. by tryptic digestion of the cell suspension followed by MALDI-TOF MS analysis"@en . "March" . "9"^^ . . "Intact cell MALDI-TOF mass spectrometry is a rapid tool for the identification and classification of microorganisms, now widely used even in clinical laboratories. However, its distinctive power is not sufficient for some closely-related species. The genus Cronobacter, formerly known as Enterobacter sakazakii, contains such species. In this work, a new method for the differentiation of five Cronobacter species is presented involving the tryptic digestion of cytoplasmatic proteins followed by MALDI mass spectrometry analysis. A database was developed for use in Bruker Biotyper software including 52 reference spectra and tested on a set of 45 samples with an overall accuracy of about 80%. The possibility of measurement automation and the short time and low cost requirements of this method compared to those of biochemical tests or PCR methods make it a supplementary option to intact cell MALDI, providing additional information about the differentiation of problematic species."@en . . .