. . "S" . "000330489200026" . . "Peptide mass mapping as an effective tool for historical mortar analysis"@en . . . "0950-0618" . "10.1016/j.conbuildmat.2013.09.059" . "[D4254669FBD0]" . "22330" . "4"^^ . . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . "7"^^ . . "GB - Spojen\u00E9 kr\u00E1lovstv\u00ED Velk\u00E9 Brit\u00E1nie a Severn\u00EDho Irska" . "January" . "Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. The second goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial." . . "4"^^ . "Crhov\u00E1, Michaela" . . "36081" . "Hrdli\u010Dkov\u00E1 Ku\u010Dkov\u00E1, \u0160t\u011Bp\u00E1nka" . "http://ac.els-cdn.com/S0950061813008994/1-s2.0-S0950061813008994-main.pdf?_tid=5723656e-97d0-11e3-978f-00000aacb361&acdnat=1392640862_a75bc3432e7e36340526b27722def713" . . "Recently, peptide mass mapping using MALDI?TOF MS (Matrix-Assisted Laser Desorption/Ionization ? Time of Flight Mass Spectrometry) was shown to be an effective tool for protein analysis in such challenging insoluble materials as historical mortars. The first aim of this study was to investigate the influence of ageing on the performance of peptide mass mapping analysis using MALDI?TOF MS. Therefore, analyses were performed on model mortar samples with different compositions after 5 years of ageing. The second goal of this work was to analyse peptide fragments using LC?MS/MS (Liquid Chromatography coupled to tandem Mass Spectrometry), which represents a new approach. The use of this method led to obtaining the sequence information that allowed for unambiguous identification of the proteins present. Our study proved the complementarity of both approaches; therefore, their combination is highly beneficial."@en . . "LC-MS/MS; MALDI-TOF MS; Peptide mass mapping method; Ageing; Protein additive; Historical mortar"@en . "Peptide mass mapping as an effective tool for historical mortar analysis" . . "RIV/60461373:22330/14:43895696" . . "RIV/60461373:22330/14:43895696!RIV15-MSM-22330___" . "Peptide mass mapping as an effective tool for historical mortar analysis"@en . "Construction and Building Materials" . . "50" . . . . "Hynek, Radovan" . . "Peptide mass mapping as an effective tool for historical mortar analysis" . . .