"22330" . . . "[89C96FB70403]" . . "2"^^ . . "Stanoven\u00ED pl\u00EDsn\u00ED metodou PCR" . "Stanoven\u00ED pl\u00EDsn\u00ED metodou PCR"@cs . "2010-01-01+01:00"^^ . "289767" . "PCR; fungi; barley; DNA; primers"@en . . "1"^^ . . . "Technologie a hodnocen\u00ED v\u00FDrobk\u016F n\u00E1pojov\u00E9ho pr\u016Fmyslu" . . "Praha" . "Stanoven\u00ED pl\u00EDsn\u00ED metodou PCR"@cs . "Praha" . "Vysok\u00E1 \u0161kola chemicko-technologick\u00E1 v Praze" . . "Fiala, Jarom\u00EDr" . "Z(MSM6046137305)" . "Detection of fungi by PCR method"@en . . . "Sladovnick\u00FD je\u010Dmen je hlavn\u00ED surovina pro v\u00FDrobu sladu a n\u00E1sledn\u011B piva. Hygienick\u00E9 po\u017Eadavky m\u016F\u017Eeme zajistit pou\u017Eit\u00EDm nezne\u010Di\u0161t\u011Bn\u00FDch surovin. Pl\u00EDs\u0148ov\u00E1 kontaminace na sladovnick\u00E9m je\u010Dmeni je obzvl\u00E1\u0161t\u011B nebezpe\u010Dn\u00E1 z hlediska produkce mykotoxin\u016F, kter\u00E9 mohou v kone\u010Dn\u00E9m v\u00FDrobku zp\u016Fsobovat vady (nap\u0159. gushing). Jednou z mo\u017Enost\u00ED ur\u010Den\u00ED pl\u00EDsn\u00ED narostl\u00FDch na je\u010Dmeni je metoda PCR. Jedn\u00E1 se o molekul\u00E1rn\u011B biologickou metodu, kdy se pracuje s DNA. Pro izolaci DNA byly pou\u017Eity izola\u010Dn\u00ED kity a samotn\u00E1 DNA byla syntetizov\u00E1na in vitro v p\u0159\u00EDstroji LightCycler? 2.0 Instrument (ROCHE). D\u00EDky vhodn\u00E9mu teplotn\u00EDmu re\u017Eimu a pou\u017Eit\u00ED spr\u00E1vn\u00FDch primer\u016F a pr\u00F3b do\u0161lo k nasyntetizov\u00E1n\u00ED DNA, kter\u00E9 se projevilo n\u00E1r\u016Fstem fluorescence." . . "8"^^ . "Detection of fungi by PCR method"@en . . "RIV/60461373:22330/10:00024308" . . . "Sladovnick\u00FD je\u010Dmen je hlavn\u00ED surovina pro v\u00FDrobu sladu a n\u00E1sledn\u011B piva. Hygienick\u00E9 po\u017Eadavky m\u016F\u017Eeme zajistit pou\u017Eit\u00EDm nezne\u010Di\u0161t\u011Bn\u00FDch surovin. Pl\u00EDs\u0148ov\u00E1 kontaminace na sladovnick\u00E9m je\u010Dmeni je obzvl\u00E1\u0161t\u011B nebezpe\u010Dn\u00E1 z hlediska produkce mykotoxin\u016F, kter\u00E9 mohou v kone\u010Dn\u00E9m v\u00FDrobku zp\u016Fsobovat vady (nap\u0159. gushing). Jednou z mo\u017Enost\u00ED ur\u010Den\u00ED pl\u00EDsn\u00ED narostl\u00FDch na je\u010Dmeni je metoda PCR. Jedn\u00E1 se o molekul\u00E1rn\u011B biologickou metodu, kdy se pracuje s DNA. Pro izolaci DNA byly pou\u017Eity izola\u010Dn\u00ED kity a samotn\u00E1 DNA byla syntetizov\u00E1na in vitro v p\u0159\u00EDstroji LightCycler? 2.0 Instrument (ROCHE). D\u00EDky vhodn\u00E9mu teplotn\u00EDmu re\u017Eimu a pou\u017Eit\u00ED spr\u00E1vn\u00FDch primer\u016F a pr\u00F3b do\u0161lo k nasyntetizov\u00E1n\u00ED DNA, kter\u00E9 se projevilo n\u00E1r\u016Fstem fluorescence."@cs . . . . "Stanoven\u00ED pl\u00EDsn\u00ED metodou PCR" . "RIV/60461373:22330/10:00024308!RIV11-MSM-22330___" . "Pet\u0159\u00EDkov\u00E1, Ad\u00E9la" . "978-80-7080-768-2" . "Malting barley is primary raw material for production malt and beer consequently. We can ensure hygienic requirements by the use of not infected raw materials. Fungal contamination on malting barley is especial dangerous by production of mycotoxins, which could cause defects (e.g. gushing) in final product. PCR is one of the choices to detect fungi in barley. It is molecular biologic method, where DNA is worked with. Isolation kits were used for isolation and DNA was syntetized in vitro in LightCycler? 2.0 Instrument (ROCHE).Thanks to appropriate temperature regime and right primers and probes DNA was synthesized. It was demonstrated by the growth of fluorescence."@en .