"Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 \u03BCg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t" . "Pulkrabov\u00E1, Jana" . "RIV/60461373:22330/10:00024297!RIV11-MSM-22330___" . . . "Nielen, M. W." . . . "6"^^ . "Food contamination caused by chemical hazards such as persistent organic pollutants (POPs) is a worldwide public health concern and requires continuous monitoring. The chromatography-based analysis methods for POPs are accurate and quite sensitive but they are time-consuming, laborious and expensive. Thus, there is a need for validated simplified screening tools, which are inexpensive, rapid, have automation potential and can detect multiple POPs simultaneously. In this study we developed a flow cytometry-based immunoassay (FCIA) using a color-encoded microbeads technology to detect benzo[a]pyrene (BaP) and other polycyclic aromatic hydrocarbons (PAHs) in buffer and food extracts as a starting point for the future development of rapid multiplex assays including other POPs in food, such as polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs). A highly sensitive assay for BaP was obtained with an IC50 of 0.3 \u03BCg L-1 using a monoclonal antibody (Mab22F12) against BaP, similar t"@en . "Haj\u0161lov\u00E1, Jana" . . "Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food"@en . "251178" . . "2"^^ . "0003-2670" . "Meimaridou, A." . . . . . "Mintzas, D." . . "7"^^ . "000279851000003" . . "RIV/60461373:22330/10:00024297" . . "1-2" . . "672" . "Polycyclic aromatic hydrocarbons; Flow cytometry; Persistent organic pollutants"@en . "Anal.Chim.Acta" . . "[1373F9DB9A64]" . "22330" . "Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food" . "P(7E08068)" . "NL - Nizozemsko" . . "Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food" . "Haasnoot, W." . "Color encoded microbeads-based flow cytometric immunoassay for polycyclic aromatic hydrocarbons in food"@en . "Noteboom, L." .