"[8C04B9835D54]" . . . . . "2"^^ . "2010-01-01+01:00"^^ . "The contribution of met region from plasmid pA81 from bacterium Achromobacter xylosoxidans A8 to heavy metal resistance"@en . "5"^^ . "P\u0159\u00EDsp\u011Bvek gen\u016F met z plasmidu pA81 bakterie Achromobacter xylosoxidans A8 k resistenci k iont\u016Fm t\u011B\u017Ek\u00FDch kov\u016F"@cs . "P\u0159edm\u011Btem studia byl region met identifikovan\u00FD na plasmidu pA81 z p\u016Fdn\u00ED bakterie achromobacter xylosoxidans A8. V tomto regonu bylo anal\u00FDzou nukleotidov\u00E9 sekvence odhaleno sedm p\u0159edpov\u011Bzen\u00FDch gen\u016F metTDYRABC. Jejich p\u0159edpov\u011Bzen\u00E9 produkty vykazuj\u00ED v\u00FDznamnou sekven\u010Dn\u00ED homologii s membr\u00E1nov\u00FDmi proteiny z rodiny Pb2+/Fe2+ transport\u00E9r\u016F (gen metT), sekund\u00E1rn\u00EDmi aktivn\u00EDmi transport\u00E9ry z CDF rodiny (metD), steroldesaturasami (metY), transkrip\u010Dn\u00EDmi regul\u00E1tory z rodiny MerR (metR), s P1-ATPasami (metA), pravd\u011Bpodobn\u00FDm membr\u00E1nov\u00FDm lipoproteinem (metB) a odpov\u00EDdaj\u00EDc\u00ED sign\u00E1ln\u00ED peptidasou prolipoprotein\u016F (metC). Za \u00FA\u010Delem studia fenotypov\u00FDch projev\u016F jednoliv\u00FDch gen\u016F met byly p\u0159ipraveny kmeny Escherichia coli GG48 nesouc\u00ED vektory na b\u00E1zi plasmidu pBla umo\u017E\u0148uj\u00EDc\u00ED konstitutivn\u00ED expresi t\u011Bchto hypotetick\u00FDch gen\u016F. V porovn\u00E1n\u00ED s kontroln\u00EDm kmenem nesouc\u00EDm samotn\u00FD plasmid pBla do\u0161lo u bun\u011Bk exprimuj\u00EDc\u00EDch metA k v\u00FDznamn\u00E9mu zv\u00FD\u0161en\u00ED rezistence v\u016F\u010Di Cd2+ a Zn2+. Exprese metA sou\u010Dasn\u011B vedla ke sn\u00ED\u017Een\u00ED mno\u017Estv\u00ED akumulovan\u00E9ho Cd" . "\u0160uman, J\u00E1chym" . . . "2"^^ . "P\u0159edm\u011Btem studia byl region met identifikovan\u00FD na plasmidu pA81 z p\u016Fdn\u00ED bakterie achromobacter xylosoxidans A8. V tomto regonu bylo anal\u00FDzou nukleotidov\u00E9 sekvence odhaleno sedm p\u0159edpov\u011Bzen\u00FDch gen\u016F metTDYRABC. Jejich p\u0159edpov\u011Bzen\u00E9 produkty vykazuj\u00ED v\u00FDznamnou sekven\u010Dn\u00ED homologii s membr\u00E1nov\u00FDmi proteiny z rodiny Pb2+/Fe2+ transport\u00E9r\u016F (gen metT), sekund\u00E1rn\u00EDmi aktivn\u00EDmi transport\u00E9ry z CDF rodiny (metD), steroldesaturasami (metY), transkrip\u010Dn\u00EDmi regul\u00E1tory z rodiny MerR (metR), s P1-ATPasami (metA), pravd\u011Bpodobn\u00FDm membr\u00E1nov\u00FDm lipoproteinem (metB) a odpov\u00EDdaj\u00EDc\u00ED sign\u00E1ln\u00ED peptidasou prolipoprotein\u016F (metC). Za \u00FA\u010Delem studia fenotypov\u00FDch projev\u016F jednoliv\u00FDch gen\u016F met byly p\u0159ipraveny kmeny Escherichia coli GG48 nesouc\u00ED vektory na b\u00E1zi plasmidu pBla umo\u017E\u0148uj\u00EDc\u00ED konstitutivn\u00ED expresi t\u011Bchto hypotetick\u00FDch gen\u016F. V porovn\u00E1n\u00ED s kontroln\u00EDm kmenem nesouc\u00EDm samotn\u00FD plasmid pBla do\u0161lo u bun\u011Bk exprimuj\u00EDc\u00EDch metA k v\u00FDznamn\u00E9mu zv\u00FD\u0161en\u00ED rezistence v\u016F\u010Di Cd2+ a Zn2+. Exprese metA sou\u010Dasn\u011B vedla ke sn\u00ED\u017Een\u00ED mno\u017Estv\u00ED akumulovan\u00E9ho Cd"@cs . "RIV/60461373:22330/10:00024257" . "P\u0159\u00EDsp\u011Bvek gen\u016F met z plasmidu pA81 bakterie Achromobacter xylosoxidans A8 k resistenci k iont\u016Fm t\u011B\u017Ek\u00FDch kov\u016F" . . "Bacterial metalloresistance; protein transportes; heavy metals"@en . "P\u0159\u00EDsp\u011Bvek gen\u016F met z plasmidu pA81 bakterie Achromobacter xylosoxidans A8 k resistenci k iont\u016Fm t\u011B\u017Ek\u00FDch kov\u016F"@cs . "Ekomonitor s.r.o." . . . "Kotrba, Pavel" . "The contribution of met region from plasmid pA81 from bacterium Achromobacter xylosoxidans A8 to heavy metal resistance"@en . . . . "P\u0159\u00EDsp\u011Bvek gen\u016F met z plasmidu pA81 bakterie Achromobacter xylosoxidans A8 k resistenci k iont\u016Fm t\u011B\u017Ek\u00FDch kov\u016F" . "RIV/60461373:22330/10:00024257!RIV11-MSM-22330___" . "P(1M06030), S, Z(MSM6046137305)" . "Inovativn\u00ED sana\u010Dn\u00ED technologie ve v\u00FDzkumu a praxi III" . . . "281966" . "Gram-negative soil bacterium Achromobacter xylosoxidans A8 hosts a 92,5-kb plasmid pA81. The analysis of nucleotide sequence revealed that the plasmid harbors a met region consisting of seven genes metTDYRAB. The homology searches suggested identities of encoded gene products as (i) putative membrane protein from Pb2+/Fe2+ family of transporters (metT gene), (ii) transporter of Cation-Diffusion Facilitator family (metD), (iii) steroldesaturase (metY), (iv) member of MerR family of metal-responsive transcriptional activators/repressors (metR), (v) efflux P1-ATPase (metA), (vi) putative membrane lipoprotein (metB) along with its cognate (vii) prolipoprotein signal peptidase (metC). In order to study the metalloresistance phenotype, which would be determined by the individual met genes, vectors based on pBla were constructed. These allowed constitutive expression of the individual met genes in E. coli GG48. The clones expressing the metA gene showed increased metalloresistance, as compared with a strain"@en . . . "22330" . . . . "Beroun" . . "978-80-86832-53-1" . "Chrudim" .