"Folia Microbiologica" . "000281277900009" . "Melzoch, Karel" . "Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum" . "Lipovsk\u00FD, Jakub" . "0015-5632" . "Linhov\u00E1, Michaela" . "Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum" . "22330" . "Paulov\u00E1, Leona" . "Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co" . . . "CZ - \u010Cesk\u00E1 republika" . . "Fribert, Petr" . . . . "55" . . "Rychtera, Mojm\u00EDr" . . "4" . . "P(FR-TI1/218), P(QH81323), S, Z(MSM6046137305)" . . "Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum"@en . . . "Clostridium pasteurianum forms acetic and butyric acids in an initial growth phase. It is a typical feature of clostridial acetone-butanol fermentation where an initial accumulation of acids is followed by production of solvents: 1-butanol, acetone and ethanol. The initiation of the solvents production coupled with endospore formation leads to decrease of the cell wall thickness. The thinner cell wall is more resistant against solvents and dyes. These changes can be observed by the method originated in Gram staining. The cell wall of G+ bacteria allows the entry of hexidium iodide (HI) and rhodamine 123 (RH123). However, the outer membrane of G- bacteria does not allow the uptake and therefore G+ bacteria are stained with higher fluorescence intensity than G- bacteria. The ratio of fluorescence intensity to forward scatter (FSC) was determined to correspond to G+ bacteria when clostridia were producing less of solvents. The significant drop of the ratio of fluorescence intensity to FSC to the level co"@en . "253945" . "Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum"@en . . . . . "7"^^ . . . . "5"^^ . . "7"^^ . . "RIV/60461373:22330/10:00023765!RIV11-MPO-22330___" . . "Flow cytometry; Gram staining; Clostridium pasteurianum; 1-butanol"@en . . "RIV/60461373:22330/10:00023765" . "Pat\u00E1kov\u00E1, Petra" . . . "[EBCEB5D698A8]" . .