"Bioprospect" . . "Transmembr\u00E1nov\u00E9 proteiny - v\u00FDzva pro hled\u00E1n\u00ED nov\u00FDch analytick\u00FDch p\u0159\u00EDstup\u016F" . . . "Transmembr\u00E1nov\u00E9 proteiny - v\u00FDzva pro hled\u00E1n\u00ED nov\u00FDch analytick\u00FDch p\u0159\u00EDstup\u016F" . . "Transmembr\u00E1nov\u00E9 proteiny - v\u00FDzva pro hled\u00E1n\u00ED nov\u00FDch analytick\u00FDch p\u0159\u00EDstup\u016F"@cs . "[2B209197B359]" . "3"^^ . . "1"^^ . "346770" . . . "Tranmembrane proteins; proteomics"@en . "RIV/60461373:22330/09:00022550" . "Transmembrane proteins - challenge for searching of new analytical approaches"@en . "Z(MSM6046137305)" . "Membr\u00E1nov\u00E9 proteiny jsou lokalizov\u00E1ny na rozhran\u00EDch r\u016Fzn\u00FDch prost\u0159ed\u00ED, a proto je jejich izolace a identifikace nezbytn\u00FDm p\u0159edpokladem pro pochopen\u00ED \u0159ady fyziologick\u00FDch proces\u016F. Extr\u00E9mn\u00ED hydrofobicita protein\u016F s transmembr\u00E1nov\u00FDmi dom\u00E9nami \u010Din\u00ED jejich anal\u00FDzu klasick\u00FDmi proteomick\u00FDmi technikami velmi komplikovanou nebo t\u00E9m\u011B\u0159 nemo\u017Enou. Proto je t\u0159eba hledat pro jejich anal\u00FDzu alternativn\u00ED p\u0159\u00EDstupy. Technika vyu\u017E\u00EDvaj\u00EDc\u00ED chromatografii na reverzn\u00ED f\u00E1zi pro redukci kontaminace rozpustn\u00FDmi proteiny a technika kombinuj\u00EDc\u00ED jednorozm\u011Brnou elektrofor\u00E9zu s kapalinovou chromatografi\u00ED spojenou s hmotnostn\u00ED spektrometri\u00ED se jev\u00ED jako slibn\u00E9 p\u0159\u00EDstupy." . "Transmembr\u00E1nov\u00E9 proteiny - v\u00FDzva pro hled\u00E1n\u00ED nov\u00FDch analytick\u00FDch p\u0159\u00EDstup\u016F"@cs . "Membr\u00E1nov\u00E9 proteiny jsou lokalizov\u00E1ny na rozhran\u00EDch r\u016Fzn\u00FDch prost\u0159ed\u00ED, a proto je jejich izolace a identifikace nezbytn\u00FDm p\u0159edpokladem pro pochopen\u00ED \u0159ady fyziologick\u00FDch proces\u016F. Extr\u00E9mn\u00ED hydrofobicita protein\u016F s transmembr\u00E1nov\u00FDmi dom\u00E9nami \u010Din\u00ED jejich anal\u00FDzu klasick\u00FDmi proteomick\u00FDmi technikami velmi komplikovanou nebo t\u00E9m\u011B\u0159 nemo\u017Enou. Proto je t\u0159eba hledat pro jejich anal\u00FDzu alternativn\u00ED p\u0159\u00EDstupy. Technika vyu\u017E\u00EDvaj\u00EDc\u00ED chromatografii na reverzn\u00ED f\u00E1zi pro redukci kontaminace rozpustn\u00FDmi proteiny a technika kombinuj\u00EDc\u00ED jednorozm\u011Brnou elektrofor\u00E9zu s kapalinovou chromatografi\u00ED spojenou s hmotnostn\u00ED spektrometri\u00ED se jev\u00ED jako slibn\u00E9 p\u0159\u00EDstupy."@cs . "Transmembrane proteins - challenge for searching of new analytical approaches"@en . . . "Membrane proteins are located on the interface between different environments. That is reason why their isolation and identification is essential for understanding of number of physiological processes. The extremely hydrophobic nature of proteins with transmembrane domains makes their analysis by classical techniques used in proteomics very complicated or nearly impossible. Thus it is necessary to seek for the alternative approaches for their analysis. Technique using chromatography on reversed phase for reduction of soluble contamination and technique using combination 1D-gel electrophoresis and liquid chromatography on reversed phase coupled to mass spectrometry seems to be very promising."@en . "1"^^ . "CZ - \u010Cesk\u00E1 republika" . . "Hynek, Radovan" . "RIV/60461373:22330/09:00022550!RIV10-MSM-22330___" . "1" . . "1210-1737" . . "22330" . . .