"Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture" . "Paulov\u00E1, Leona" . . . "4"^^ . . . "Produkce rekombinantn\u00EDch protein\u016F bu\u0148kami Picha pastoris Mut+ rostouc\u00EDmi v kontinu\u00E1ln\u00ED kultu\u0159e"@cs . "390086" . . . . . "Vil\u010Dkov\u00E1, Stanislava" . . "Dalian Institute of Chemical Physics" . . . "The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01 g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, the specific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the higher carbon sour" . . "Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture"@en . . "[74D24A106ACB]" . "Dalian" . . . "The growth kinetic, overproduction of excreted recombinant protein and cell physiology of Pichia pastoris Mut+ strain were studied on mixed substrate containing two carbon sources (glucose and methanol) in chemostat. Although glucose is considered as a repressive substrate for AOX promoter, recombinant trypsinogen was expressed when glucose was used in mixture with methanol in substrate limited culture. The specific methanol uptake rate was rising with increasing dilution rate reaching maximum 0.090.01 g.gCDW-1.h-1 at 0.15 h-1; both co-substrates were utilised simultaneously and fully. At dilution rates, exceeding 0.15 h-1 the specific methanol uptake rate was decreasing and a residual methanol was detected in the culture broth. On the other hand, the specific glucose uptake rate was increasing within the interval of measured dilution rates reaching maximum (0.240.01) g.gCDW-1.h-1 at 0.2 h-1. In case of continuous cultures performed at higher dilution rates the yeasts preferred the higher carbon sour"@en . "2008-10-12+02:00"^^ . . . . . . "Rychtera, Mojm\u00EDr" . "Heterologous protein; Pichia pastoris Continuous culture; Yeast physiology; Flow cytometry"@en . "0168-1656" . "22330" . "5"^^ . "P(1P05OE189), Z(MSM6046137305)" . "Produkce rekombinantn\u00EDch protein\u016F bu\u0148kami Picha pastoris Mut+ rostouc\u00EDmi v kontinu\u00E1ln\u00ED kultu\u0159e"@cs . "Proceedings of the 13th International Biotechnology Symposium and Exhibition" . "RIV/60461373:22330/08:00021237" . . "2"^^ . "RIV/60461373:22330/08:00021237!RIV09-MSM-22330___" . "Bransk\u00E1, Barbora" . "Kinetika r\u016Fstu, nadprodukce rekombinantn\u00EDho proteinu a fyziologie kvasinek Pichia pastoris Mut+ byly sledov\u00E1na na sm\u011Bsn\u00E9m substr\u00E1tu obsahuj\u00EDc\u00EDm 2 zdroje uhl\u00EDku (glukosu a methanol) rostouc\u00ED v kontinu\u00E1ln\u00ED kultu\u0159e. Specific\u00E1 rychlost spot\u0159eby methanolu vzr\u016Fstala se zvy\u0161uj\u00EDc\u00ED se z\u0159e\u010Fovac\u00ED rychlost\u00ED, maximum 0.090.01 g.gCDW-1.h-1 bylo dosa\u017Eeno p\u0159i D = 0.15 h-1; oba ko-substraty byly utilizov\u00E1ny sou\u010Dasn\u011B a zcela. Maxim\u00E1ln\u00ED koncentrace trypsinogenu 145 mg.L-1 a maxim\u00E1ln\u00ED specifick\u00E1 productivita (1.17 mg.gCDW-1.h-1) byly dosa\u017Eeny p\u0159i D = 0.13 h-1."@cs . "Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture" . . "Melzoch, Karel" . "Dalian, China" . "Production of heterologous protein by Pichia pastoris Mut+ strain grown in continuous culture"@en .