"2"^^ . . . . "P(GA303/08/0958), Z(MSM6046137305)" . "RIV/60461373:22330/08:00019961" . "Lap\u010D\u00EDk, Old\u0159ich" . "Salamanca" . "Buk\u00E1\u010Dkov\u00E1, Lucie" . "Salamanca" . "University of Salamanca" . . . "22330" . . . "Polyphenols Communications" . "952-10-1977-8" . . "Equol is estrogenic isoflavan, which is formed by the action of intestinal microflora from daidzein (an isoflavone) present in different beverages of leguminous origin. The ability of individual subjects to convert daidzein to equol is highly variable. It has been assumed that equol may have higher biological activity than its precursor. High urinary excretion of equol has been suggested to be associated with a reduction in breast cancer risk and the menopause associated osteoporosis. Here we present the development of a competitive enzyme-linked immunosorbent assay (ELISA) method for the determination of equol in human body fluids. Isoflavone conjugates coupled to bovine serum albumin at positions 7-O- and 4\u00B4-O- via a carboxymethyl spacer were used as immunogens. Conjugates of the same haptens with ovalbumin have been synthesized as coating antigens. Assay?s conditions, including concentrations of antisera and coating antigens, were optimized and the effect of reaction time was determined, various" . "Equol je fyziologicky v\u00FDznamn\u00FD metabolit vznikaj\u00EDc\u00ED p\u016Fsoben\u00EDm dosud neidentifikovan\u00FDch st\u0159evn\u00EDch bakteri\u00ED na daidzein a formononetin ? isoflavony vyskytuj\u00EDc\u00ED se v soje a dal\u0161\u00EDch bobovit\u00FDch rostlin\u00E1ch. Vykazuje estrogenn\u00ED aktivitu, kter\u00E1 je dokonce siln\u011Bj\u0161\u00ED ne\u017E u jeho prekursor\u016F. P\u0159em\u011Bna daidzeinu na equol prob\u00EDh\u00E1 pouze u 30-50% lidsk\u00E9 populace. V t\u00E9to studii jsme vyvinuli metody nep\u0159\u00EDm\u00E9 kompetitivn\u00ED ELISA pro stanoven\u00ED equolu v biologick\u00E9m materi\u00E1lu (krev, mo\u010D). Jako imunogeny byly pou\u017Eity konjug\u00E1ty equolu s hov\u011Bz\u00EDm s\u00E9rov\u00FDm albuminem v\u00E1zan\u00E9 na nosi\u010D prost\u0159ednictv\u00EDm karboxymethyletherov\u00FDch m\u016Fstk\u016F v poloh\u00E1ch C7 a C4?. Konjug\u00E1ty stejn\u00FDch hapten\u016F s ovalbuminem byly pou\u017Eity k imobilizaci. Jednotliv\u00E9 ELISA metody byly optimalizov\u00E1ny testov\u00E1n\u00EDm r\u016Fzn\u00FDch koncentrac\u00ED protil\u00E1tek a potahovac\u00EDch antigen\u016F, up\u0159esn\u011Bn\u00EDm reak\u010Dn\u00EDho \u010Dasu a vyu\u017Eit\u00EDm blokovac\u00EDch \u010Dinidel. K\u0159\u00ED\u017Eov\u00E9 reakce jednotliv\u00FDch imunoanalytick\u00FDch metod s dal\u0161\u00EDmi isoflavony jsou v p\u0159ijateln\u00E9m rozmez\u00ED (do 0.6%) a ovlin\u011Bn\u00ED molekulami s jinou strukturou j"@cs . . . "ELISA method for estimation of equol"@en . . "RIV/60461373:22330/08:00019961!RIV09-MSM-22330___" . "366000" . . "Equol is estrogenic isoflavan, which is formed by the action of intestinal microflora from daidzein (an isoflavone) present in different beverages of leguminous origin. The ability of individual subjects to convert daidzein to equol is highly variable. It has been assumed that equol may have higher biological activity than its precursor. High urinary excretion of equol has been suggested to be associated with a reduction in breast cancer risk and the menopause associated osteoporosis. Here we present the development of a competitive enzyme-linked immunosorbent assay (ELISA) method for the determination of equol in human body fluids. Isoflavone conjugates coupled to bovine serum albumin at positions 7-O- and 4\u00B4-O- via a carboxymethyl spacer were used as immunogens. Conjugates of the same haptens with ovalbumin have been synthesized as coating antigens. Assay?s conditions, including concentrations of antisera and coating antigens, were optimized and the effect of reaction time was determined, various"@en . "equol; phytoestrogen; ELISA"@en . "1"^^ . "Al-Maharik, Nawaf" . . "ELISA method for estimation of equol" . . "ELISA method for estimation of equol" . . . "ELISA pro stanoven\u00ED equolu"@cs . "4"^^ . "ELISA method for estimation of equol"@en . . "ELISA pro stanoven\u00ED equolu"@cs . "[70ED7E8073E7]" . "Lankov\u00E1, Petra" . . "2008-11-08+01:00"^^ .