"Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con"@en . "Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS"@en . . . "6"^^ . . "\u0160antr\u016F\u010Dek, Ji\u0159\u00ED" . . "RIV/60461373:22330/07:00019275" . "Fractionation of Co, Cu, Fe, Mn, Mo, Ni, P and Zn species in extracts of buckwheat semolina and amaranth flour in 0.02 moll-1 Tris-HCl (pH=7.5) buffer was performed by SEC/ICP-MS hyphenation. Element specific chromatograms of the buckwheat extract are similar to those of legumes and cereals. Except for Fe and Mn the remaining metals are concentrated in a low-molecular fraction (Mr<2 kDa). Fe is bound in a high-molecular fraction (Mr>150 kDa). Chromatograms of Co, Fe, Mo and Ni species in amaranth extracts correspond with this pattern too. However, Cu is also bound in a medium-molecular fraction (Mr~40 kDa) and in a high-molecular one (Mr>150 kDa). The main part of Mn in both extracts is present in labile complex and ionic form. Fractions rich of trace metals were isolated using preparative-scale SEC and the organic ligands of metals were consequently preconcentrated on Chelex 100 in Cu2+ form and eluted by ammonia solution. Low content of sulphur containing amino acids (Cys, Met) and high con" . . "Kom\u00EDnkov\u00E1, Jana" . "Z(MSM6046137305)" . . . "409461" . "895-904" . "Anal\u00FDza frakc\u00ED prvkov\u00FDch speci\u00ED v pohankov\u00E9 a amarantov\u00E9 mouce metodami SEC/ICP-MS a MALDI-MS"@cs . . "Pol\u00E1k, Jan" . "Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS"@en . . "Anal\u00FDza frakc\u00ED prvkov\u00FDch speci\u00ED v pohankov\u00E9 a amarantov\u00E9 mouce metodami SEC/ICP-MS a MALDI-MS"@cs . "Amaranth; buckwheat; speciation; trace elements; MALDI-MS; ICP-MS"@en . "[26AAE12DF124]" . "European Food Research and Technology" . . "Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS" . . "Kod\u00ED\u010Dek, Milan" . . "RIV/60461373:22330/07:00019275!RIV08-MSM-22330___" . "Mestek, Oto" . "DE - Spolkov\u00E1 republika N\u011Bmecko" . . . . "Analysis of elements species fractions in buckwheat and amaranth flours by SEC/ICP-MS and MALDI-MS" . "5-6" . . . "10"^^ . "Kopl\u00EDk, Richard" . "22330" . "Frakcionace speci\u00ED Co, Cu, Fe, Mn, Mo, Ni, P a Zn v extraktech pohankov\u00E9 krupice a amarantov\u00E9 mouky v 0,02 mol/l Tris-HCL (pH=7,5) byla provedena metodou SEC/ICP-MS."@cs . "Kvasni\u010Dka, Franti\u0161ek" . . "1438-2377" . . . "225" . . . . "7"^^ .