"RIV/60461373:22330/07:00019139!RIV08-MSM-22330___" . "Hul\u00EDn, Petr" . . "5"^^ . . . "Hochel, Igor" . "ELISA; determination; prolamins; barley"@en . . . "440219" . "[D11DA0688AF7]" . . . "Pr\u00E1ce se zab\u00FDv\u00E1 mo\u017Enostmi imunochemick\u00E9 detekce hordeinov\u00E9 frakce b\u00EDlkovin je\u010Dmene a p\u0159\u00EDpadn\u00FDch \u0161t\u011Bpn\u00FDch deriv\u00E1t\u016F, respektive charakterizac\u00ED monoklon\u00E1ln\u00EDch protil\u00E1tek p\u0159ipraven\u00FDch za \u00FA\u010Delem v\u00FDvoje rychl\u00E9ho, specifick\u00E9ho a citliv\u00E9ho stanoven\u00ED hordeinov\u00FDch b\u00EDlkovin v potravin\u00E1\u0159stv\u00ED, konkr\u00E9tn\u011B v pivu. Byla vyvinuta metoda nep\u0159\u00EDm\u00E9 kompetitivn\u00ED ELISA prolamin\u016F je\u010Dmene a stanoveny pracovn\u00ED koncentrace v\u0161ech imunoreagenci\u00ED. D\u00E1le byly testov\u00E1ny k\u0159\u00ED\u017Eov\u00E9 interakce jednotliv\u00FDch monoklon\u00E1ln\u00EDch protil\u00E1tek s proteiny ostatn\u00EDch cere\u00E1li\u00ED. Detek\u010Dn\u00ED limit metody \u010Dinil 2 ppm, p\u0159i\u010Dem\u017E line\u00E1rn\u00ED rozsah kalibra\u010Dn\u00ED k\u0159ivky se pohybuje v rozmez\u00ED 1-100 ppm." . "Sborn\u00EDk XXXVIII. symposium o nov\u00FDch sm\u011Brech v\u00FDroby a hodnocen\u00ED potravin, , 21.-23.5. 2007, Skalsk\u00FD Dv\u016Fr u Byst\u0159ice nad Pern\u0161tejnem, \u010CR" . "Dost\u00E1lek, Pavel" . "2"^^ . . . "RIV/60461373:22330/07:00019139" . . . "227-231" . "Otimalizace ELISA metody pro stanoven\u00ED prolamin\u016F je\u010Dmene" . . "Pr\u00E1ce se zab\u00FDv\u00E1 mo\u017Enostmi imunochemick\u00E9 detekce hordeinov\u00E9 frakce b\u00EDlkovin je\u010Dmene a p\u0159\u00EDpadn\u00FDch \u0161t\u011Bpn\u00FDch deriv\u00E1t\u016F, respektive charakterizac\u00ED monoklon\u00E1ln\u00EDch protil\u00E1tek p\u0159ipraven\u00FDch za \u00FA\u010Delem v\u00FDvoje rychl\u00E9ho, specifick\u00E9ho a citliv\u00E9ho stanoven\u00ED hordeinov\u00FDch b\u00EDlkovin v potravin\u00E1\u0159stv\u00ED, konkr\u00E9tn\u011B v pivu. Byla vyvinuta metoda nep\u0159\u00EDm\u00E9 kompetitivn\u00ED ELISA prolamin\u016F je\u010Dmene a stanoveny pracovn\u00ED koncentrace v\u0161ech imunoreagenci\u00ED. D\u00E1le byly testov\u00E1ny k\u0159\u00ED\u017Eov\u00E9 interakce jednotliv\u00FDch monoklon\u00E1ln\u00EDch protil\u00E1tek s proteiny ostatn\u00EDch cere\u00E1li\u00ED. Detek\u010Dn\u00ED limit metody \u010Dinil 2 ppm, p\u0159i\u010Dem\u017E line\u00E1rn\u00ED rozsah kalibra\u010Dn\u00ED k\u0159ivky se pohybuje v rozmez\u00ED 1-100 ppm."@cs . . . "3"^^ . "The work deals with the possibilities of immunochemical detection of hordein fractions of barley proteins and possible cleavage derivates, or more precisely by characterization of monoclonal antibodies prepared for the purpose of a rapid, specific and sensitive determination of hordein proteins in the food industry, specifically in beer. A method of indirect competitive ELISA for barley prolamins has been developed and working concentrations of all immunoagents have been determined. Further to this, cross interactions of particular monoclonal antibodies with the proteins of the other cereals have been tested. The detection limit of the method was 2 ppm with the linear range of the calibration curve ranging from 1 to 100 ppm."@en . . "22330" . . "Otimalizace ELISA metody pro stanoven\u00ED prolamin\u016F je\u010Dmene"@cs . . "P(1M0570), Z(MSM6046137305)" . . "Otimalizace ELISA metody pro stanoven\u00ED prolamin\u016F je\u010Dmene"@cs . "Otimalizace ELISA metody pro stanoven\u00ED prolamin\u016F je\u010Dmene" . "V\u00DAPP" . "Praha" . "Opimization of ELISA Metod for determination of barley prolamins"@en . "1802-1433" . . "Opimization of ELISA Metod for determination of barley prolamins"@en .