. "Olomouc" . "Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8"@en . "Plasmid pA81 z kmene Achromobacter xylosoxidans A8, kter\u00FD degraduje chlorbenzo\u00E1ty"@cs . . . "Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8" . "RIV/60461373:22330/04:00012857" . "Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8"@en . "Univerzita Palack\u00E9ho v Olomouci" . "[E0E4F5B01453]" . "1"^^ . "Chodora, Zden\u011Bk" . "2004-01-01+01:00"^^ . . "P(LN00A079)" . "Plasmid pA81 z kmene Achromobacter xylosoxidans A8, kter\u00FD degraduje chlorbenzo\u00E1ty"@cs . . . . "The Achromobacter xylosoxidans strain A8 was isolated in the Czech Republic from PCB contaminated soil. It is able to use 2-clorobenzoate (2-CB) and 2,5-dichlorobenzoate (2,5-DCB) as sole sources of carbon and energy. We analyze the A. xylosoxidans A8 with the aim to characterize genes involved in the degradation of CBs.The genome of this strain contains two large conjugative plasmids pA81 (~ 100 kbp) and pA82 (~ 250 kbp). The genes encoding enzymes involved in the degradation pathway were found to be situated directly on the plasmid pA81. On the basis of the pA81 sequence analysis we identified clusters responsible for the plasmid replication, conjugative transfer, stable inheritance, degradation of xenobiotics and transposition. Comparison of the plasmid %22backbone%22 constituted by regions of replication control and stability and two Tra1/Tra2 regions responsible for conjugative transfer shows the highest similarity to the backbone of IncP\u00DF plasmids. Members of this plasmid group can replica" . "22330" . "Proceedings of the XIX. meeting of czech and slovak societies for biochemistry and molecula r biology" . "Jen\u010Dov\u00E1, V\u011Bra" . "0232-0061" . . . "Strnad, Hynek" . "The Achromobacter xylosoxidans strain A8 was isolated in the Czech Republic from PCB contaminated soil. It is able to use 2-clorobenzoate (2-CB) and 2,5-dichlorobenzoate (2,5-DCB) as sole sources of carbon and energy. We analyze the A. xylosoxidans A8 with the aim to characterize genes involved in the degradation of CBs.The genome of this strain contains two large conjugative plasmids pA81 (~ 100 kbp) and pA82 (~ 250 kbp). The genes encoding enzymes involved in the degradation pathway were found to be situated directly on the plasmid pA81. On the basis of the pA81 sequence analysis we identified clusters responsible for the plasmid replication, conjugative transfer, stable inheritance, degradation of xenobiotics and transposition. Comparison of the plasmid %22backbone%22 constituted by regions of replication control and stability and two Tra1/Tra2 regions responsible for conjugative transfer shows the highest similarity to the backbone of IncP\u00DF plasmids. Members of this plasmid group can replica"@en . . . . . . "Pa\u010Des, V\u00E1clav" . "4"^^ . "579700" . "RIV/60461373:22330/04:00012857!RIV/2005/MSM/223305/N" . "plasmid pA81"@en . "Plasmid pA81 z kmene Achromobacter xylosoxidans A8, kter\u00FD degraduje chlorbenzoaty"@cs . . "Plasmid pA81 from chlorobenzoate degrading strain Achromobacter xylosoxidans A8" . "4"^^ . "228" . . . "Olomouc" .