. . . . "Increased serum levels of resistin, a molecule secreted by fat cells, have been proposed as a possible mechanistic link between obesity and insulin resistance. To further investigate the effects of resistin on glucose metabolism, we derived a novel transgenic strain of spontaneously hypertensive rats expressing the mouse resistin gene under the control of the fat-specific aP2 promoter and also performed in vitro studies of the effects of recombinant resistin on glucose metabolism in isolated skeletal muscle. Expression of the resistin transgene was detected by Northern blot analysis in adipose tissue and by real-time PCR in skeletal muscle and was associated with increased serum fatty acids and muscle triglycerides, impaired skeletal muscle glucose metabolism, and glucose intolerance in the absence of any changes in serum resistin concentrations. In skeletal muscle isolated from non-transgenic spontaneously hypertensive rats, in vitro incubation with recombinant resistin significantly inhibited insuli"@en . "0"^^ . "Proteins; Muscle; Genetic engineering; Living systems studies; Cells; Oils and fats; Insulin; Metabolism; Glucose; Genes; Fatty acids;"@en . "0"^^ . "631338" . "22330" . . "Increased serum levels of resistin, a molecule secreted by fat cells, have been proposed as a possible mechanistic link between obesity and insulin resistance. To further investigate the effects of resistin on glucose metabolism, we derived a novel transgenic strain of spontaneously hypertensive rats expressing the mouse resistin gene under the control of the fat-specific aP2 promoter and also performed in vitro studies of the effects of recombinant resistin on glucose metabolism in isolated skeletal muscle. Expression of the resistin transgene was detected by Northern blot analysis in adipose tissue and by real-time PCR in skeletal muscle and was associated with increased serum fatty acids and muscle triglycerides, impaired skeletal muscle glucose metabolism, and glucose intolerance in the absence of any changes in serum resistin concentrations. In skeletal muscle isolated from non-transgenic spontaneously hypertensive rats, in vitro incubation with recombinant resistin significantly inhibited insuli" . . . "7"^^ . "278" . "P(GA301/03/0751)" . "0021-9258" . . . . "RIV/60461373:22330/03:00008965!RIV/2004/GA0/223304/N" . "Wang, Jiaming" . . . "Jansa, Petr" . . "Pravenec, Michal" . "US - Spojen\u00E9 st\u00E1ty americk\u00E9" . . "The Journal of biological chemistry" . . "[607FF59A434B]" . . . . "8"^^ . "Mlejnek, Petr" . "Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat"@en . "RIV/60461373:22330/03:00008965" . . "Landa, Vladimir" . "46" . "Kurtz, Theodore" . "Z\u00EDdek, V\u00E1clav" . "Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat"@en . "Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat" . "Kazdov\u00E1, Ludmila" . . "45209-15" . "1"^^ . . "Transgenic Resistin Impair skeletal Muscle Glucose Metabolism in the Spontaneosly Hypertensive Rat" . . .